Enhanced resistance to coldwater disease following feeding of probiotic bacterial strains to rainbow trout (Oncorhynchus mykiss)
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Burbank, DR and Shah, DH and LaPatra, SE and Fornshell, G and Cain, KD, Enhanced resistance to coldwater disease following feeding of probiotic bacterial strains to rainbow trout (Oncorhynchus mykiss), Aquaculture: An International Journal Devoted to Fundamental Aquatic Food Resources, 321, (3-4) pp. 185-190. ISSN 0044-8486 (2011) [Refereed Article]
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a b s t r a c t
Received 24 June 2011
Received in revised form 29 August 2011
Accepted 2 September 2011
Available online 10 September 2011
Flavobacterium psychrophilum is the causative agent of coldwater disease (CWD), also called rainbow trout fry
syndrome (RTFS), and has a major impact on salmonid aquaculture worldwide. The aim of this study was to
evaluate the efficacy of non-pathogenic probiotic bacterial strains to reduce mortality due to F. psychrophilum
infection in rainbow trout, (Oncorhynchus mykiss). Candidate probiotics, previously demonstrated to have in
vitro inhibition towards F. psychrophilum and the potential to survive in the gastrointestinal (GI) tract, were
administered through oil-dressed feed at 106 to 108 bacterial cells g−1, prior to and/or after infection with F.
psychrophilum. Administration of two of the ten probiotic strains (C6-6 and C6-8), resulted in a significant reduction
in mortality due to F. psychrophilum infection (Pb0.05) when compared to control fish. Both probiotic
strains were consistently reisolated from the GI tract indicating their ability to persistently colonize the GI
tract. The 16S rRNA sequences from strains C6-6 and C6-8 indicated that these strains were distinct from
each other but shared 99.8% similarity with Enterobacter amnigenus and Enterobacter strain PIC15, respectively.
Results from this study demonstrate the potential for these probiotic strains to provide benefit and an alternative
strategy for managing F. psychrophilum infections in fish in aquaculture.
© 2011 Elsevier B.V. All rights reserved.
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