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Neural progenitor number is regulated by nuclear factor-kappa B p65 and p50 subunit-dependent proliferation rather than cell survival

Citation

Young, KM and Bartlett, PF and Coulson, EJ, Neural progenitor number is regulated by nuclear factor-kappa B p65 and p50 subunit-dependent proliferation rather than cell survival, Journal of Neuroscience Research, 83, (1) pp. 39-49. ISSN 0360-4012 (2006) [Refereed Article]

DOI: doi:10.1002/jnr.20702

Abstract

The number of cells generated by a proliferating stem or precursor cell can be influenced both by proliferation and by the degree of cell death/survival of the progeny generated. In this study, the extent to which cell survival controls progenitor number was examined by comparing the growth characteristics of neurosphere cultures derived from mice lacking genes for the death-inducing Bcl-2 homologue Hara Kiri (Hrk), apoptosis-associated protein 1 (Apaf1), or the prosurvival nuclear factor-κB (NFκB) subunits p65, p50, or c-rel. We found no evidence that Hrk or Apaf1, and by inference the mitochondrial cell death pathway, are involved in regulating the number of neurosphere-derived progeny. However, we identified the p65p50 NFκB dimer as being required for the normal growth and expansion of neurosphere cultures. Genetic loss of both p65 and p50 NFκB subunits resulted in a reduced number of progeny but an increased proportion of neurons. No effect on cell survival was observed. This suggests that the number and fate of neural progenitor cells are more strongly regulated by cell cycle control than survival. © 2005 Wiley-Liss, Inc.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Cellular Nervous System
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Young, KM (Dr Kaylene Young)
ID Code:73678
Year Published:2006
Web of Science® Times Cited:20
Deposited By:Research Division
Deposited On:2011-10-21
Last Modified:2011-10-21
Downloads:0

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