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Neural progenitor number is regulated by nuclear factor-kappa B p65 and p50 subunit-dependent proliferation rather than cell survival

journal contribution
posted on 2023-05-17, 08:36 authored by Kaylene YoungKaylene Young, Bartlett, PF, Coulson, EJ
The number of cells generated by a proliferating stem or precursor cell can be influenced both by proliferation and by the degree of cell death/survival of the progeny generated. In this study, the extent to which cell survival controls progenitor number was examined by comparing the growth characteristics of neurosphere cultures derived from mice lacking genes for the death-inducing Bcl-2 homologue Hara Kiri (Hrk), apoptosis-associated protein 1 (Apaf1), or the prosurvival nuclear factor-κB (NFκB) subunits p65, p50, or c-rel. We found no evidence that Hrk or Apaf1, and by inference the mitochondrial cell death pathway, are involved in regulating the number of neurosphere-derived progeny. However, we identified the p65p50 NFκB dimer as being required for the normal growth and expansion of neurosphere cultures. Genetic loss of both p65 and p50 NFκB subunits resulted in a reduced number of progeny but an increased proportion of neurons. No effect on cell survival was observed. This suggests that the number and fate of neural progenitor cells are more strongly regulated by cell cycle control than survival. © 2005 Wiley-Liss, Inc.

History

Publication title

Journal of Neuroscience Research

Volume

83

Pagination

39-49

ISSN

0360-4012

Department/School

Menzies Institute for Medical Research

Publisher

Wiley-Liss

Place of publication

Div John Wiley & Sons Inc, 605 Third Ave, New York, USA, Ny, 10158-0012

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the biological sciences

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