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SOCS3 negatively regulates LIF signaling in neural precursor cells
journal contribution
posted on 2023-05-17, 08:36 authored by Emery, B, Merson, TD, Snell, C, Kaylene YoungKaylene Young, Ernst, M, Kilpatrick, TJCytokines that signal through the LIFRβ/gp130 receptor complex, including LIF and CNTF, promote the self-renewal of embryonic and adult neural precursor cells (NPCs). In non-CNS tissues, the protein suppressor of cytokine signaling-3 (SOCS3) negatively regulates signaling through gp130. Here, we analyze the role of SOCS3 in inhibiting LIF signaling in NPCs in vitro. SOCS3 is rapidly expressed by NPCs in response to LIF stimulation, with this expression largely dependent on recruitment of STAT proteins to the activated gp130 receptor. Proliferating NPC cultures can be generated from SOCS3 knockout (SOCS3KO/KO) embryos and display prolonged STAT3 phosphorylation and induction of the GFAP gene in response to LIF. In comparison with SOCS3 wild-type (SOCS3WT/WT) NPCs, SOCS3KO/KO cultures display enhanced self-renewal capacity. However, the clonal potential of SOCS3 WT/WT but not SOCS3KO/KO NPCs is enhanced by exogenous LIF. Thus, SOCS3 acts as a negative regulator of LIF signaling in NPCs. © 2006 Elsevier Inc. All rights reserved.
History
Publication title
Molecular and Cellular NeurosciencesVolume
31Issue
4Pagination
739-747ISSN
1044-7431Department/School
Menzies Institute for Medical ResearchPublisher
Academic Press Inc Elsevier SciencePlace of publication
525 B St, Ste 1900, San Diego, USA, Ca, 92101-4495Repository Status
- Restricted