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An Fgfr3-iCreER(T2) Transgenic Mouse Line for Studies of Neural Stem Cells and Astrocytes

Citation

Young, KM and Mitsumori, T and Pringle, N and Grist, M and Kessaris, N and Richardson, WD, An Fgfr3-iCreER(T2) Transgenic Mouse Line for Studies of Neural Stem Cells and Astrocytes, Glia, 58, (8) pp. 943-953. ISSN 0894-1491 (2010) [Refereed Article]

DOI: doi:10.1002/glia.20976

Abstract

The lack of markers for astrocytes, particularly gray matter astrocytes, significantly hinders research into their development and physiological properties. We previously reported that fibroblast growth factor receptor 3 (Fgfr3) is expressed by radial precursors in the ventricular zone of the embryonic neural tube and subsequently by differentiated astrocytes in gray and white matter. Here, we describe an Fgfr3-iCreERT2 phage artificial chromosome transgenic mouse line that allows efficient tamoxifen-induced Cre recombination in Fgfr3-expressing cells, including radial glial cells in the embryonic neural tube and both fibrous and protoplasmic astrocytes in the mature central nervous system. This mouse strain will therefore be useful for studies of normal astrocyte biology and their responses to CNS injury or disease. In addition, Fgfr3-iCreERT2 drives Cre recombination in all neurosphere-forming stem cells in the adult spinal cord and at least 90% of those in the adult forebrain subventricular zone. We made use of this to show that there is continuous accumulation of all major interneuron subtypes in the olfactory bulb (OB) from postnatal day 50 (P50) until at least P230 (∼8 months of age). It therefore seems likely that adult-born interneurons integrate into existing circuitry and perform long-term functions in the adult OB. 2010 Wiley-Liss, Inc.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Cellular Nervous System
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Young, KM (Dr Kaylene Young)
ID Code:73672
Year Published:2010
Web of Science® Times Cited:54
Deposited By:Research Division
Deposited On:2011-10-21
Last Modified:2011-10-21
Downloads:0

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