eCite Digital Repository

Assessment of snapper (Pagrus auratus) natural IgM binding to bromelain treated sheep erythrocytes


Morrison, RN and Lyons, AB and Nowak, BF and Hayball, JD, Assessment of snapper (Pagrus auratus) natural IgM binding to bromelain treated sheep erythrocytes, Fish and Shellfish Immunology, 18 pp. 91-99. ISSN 1050-4648 (2005) [Refereed Article]

DOI: doi:10.1016/j.fsi.2004.06.006


Normal snapper (Pagrus auratus Bloch and Schneider) serum was examined for natural IgM that binds to protease (bromelain) treated sheep erythrocytes (BrSRBC) in a model assay system that has been used to appraise natural IgM of various mammals. Normal snapper serum lysed BrSRBC while haemolysis was abrogated by heat inactivation of serum and in divalent cation-deficient conditions, indicative of classical complement mediated lysis. In addition, heat inactivated normal snapper serum agglutinated BrSRBC while phosphatidylcholine (PtC) liposomes partially inhibited both haemolysis and agglutination. Inhibition of haemolysis and agglutination may have been mediated by an interaction between immunoglobulin (Ig) and PtC as protein A purified snapper Ig bound to PtC liposomes. However it is not known if this binding was PtC specific nor if the binding was initiated by either the Fab and/or Fc domains of snapper Ig. BrSRBC plaque forming cells (PFC) were detected in the peritoneal cavity, spleen, head kidney and peripheral blood of normal snapper. The greatest proportion of BrSRBC PFC per B cell was within the peritoneal cavity followed by the spleen, peripheral blood and head kidney. Together, these data suggest that normal snapper serum may contain natural Ig that binds BrSRBC, activating the classical complement cascade

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Zoology
Research Field:Animal Immunology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Agricultural and Veterinary Sciences
UTAS Author:Lyons, AB (Associate Professor Bruce Lyons)
ID Code:73620
Year Published:2005
Web of Science® Times Cited:4
Deposited By:Medicine
Deposited On:2011-10-19
Last Modified:2011-12-06

Repository Staff Only: item control page