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Improved electrotransformation frequencies of Corynebacterium glutamicum using cell-surface mutants

Citation

Jang, KH and Britz, ML, Improved electrotransformation frequencies of Corynebacterium glutamicum using cell-surface mutants, Biotechnology Letters, 22, (7) pp. 539-545. ISSN 0141-5492 (2000) [Refereed Article]

DOI: doi:10.1023/A:1005629224109

Abstract

The electrotransformation efficiency for homologously- and heterologously-derived plasmid DNA was determined for two families of Corynebacterium glutamicum strains derived from ATCC13059 (AS019 auxotrophic, cell surface mutants MLB133 and MLB194) and ATCC13032 (parent strain and restriction-minus mutants RM3 and RM4), following their growth in LBG supplemented with glycine plus isonicotinic acid hydrazide (INH). Electrotransformation efficiencies of MLB 133 were up to 100-fold higher than for strain ASO19 and, when using heterologously-derived plasmid DNA, MLB133 showed efficiencies comparable to or better than strains RM3 and RM4, demonstrating the relative importance of cell surface structures in impeding DNA uptake in C. glutamicum. Transmission electron microscopy analysis of cell surface structures showed that strain MLB133 has a thin cell wall relative to AS019 and growth in either glycine or INH further diminished its thickness. Both RM3 and RM4 were more sensitive to INH than ATCC13032 and growth in glycine plus INH further improved transformation efficiency. The mycolic acid composition of these strains is described and the impact of glycine and INH on this is reported.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Bacteriology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Britz, ML (Professor Margaret Britz)
ID Code:73312
Year Published:2000
Web of Science® Times Cited:4
Deposited By:Research Division
Deposited On:2011-09-26
Last Modified:2011-09-26
Downloads:0

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