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Degradation and mineralization of high-molecular-weight polycyclic aromatic hydrocarbons by defined fungal-bacterial cocultures

Citation

Boonchan, S and Britz, ML and Stanley, GA, Degradation and mineralization of high-molecular-weight polycyclic aromatic hydrocarbons by defined fungal-bacterial cocultures, Applied and Environmental Microbiology, 66, (3) pp. 1007-1019. ISSN 0099-2240 (2000) [Refereed Article]

DOI: doi:10.1128/AEM.66.3.1007-1019.2000

Abstract

This study investigated the biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons (PAHs) in liquid media and soil by bacteria (Stenotrophomonas maltophilia VUN 10,010 and bacterial consortium VUN 10,009) and a fungus (Penicillium janthinellum VUO 10,201) that were isolated from separate creosote- and manufactured-gas plant-contaminated soils. The bacteria could use pyrene as their sole carbon and energy source in a basal salts medium (BSM) and mineralized significant amounts of benzo[a]pyrene cometabolically when pyrene was also present in BSM. P. janthinellum VUO 10,201 could not utilize any high-molecular-weight PAH as sole carbon and energy source but could partially degrade these if cultured in a nutrient broth. Although small amounts of chrysene, benz[a]anthracene, benzo[a]pyrene, and dibenz[a,h]anthracene were degraded by axenic cultures of these isolates in BSM containing a single PAH, such conditions did not support significant microbial growth or PAIl mineralization. However, significant degradation of, and microbial growth on, pyrene, chrysene, benz[a] anthracene, benzo[a] pyrene, and dibenz[a,h] anthracene, each as a single PAIl in BSM, occurred when P. janthinellum VUO 10,201 and either bacterial consortium VUN 10,009 or S. maltophilia VUN 10,010 were combined in the one culture, i.e., fungal- bacterial cocultures: 25% of the benzo[a]pyrene was mineralized to CO2 by these cocultures over 49 days, accompanied by transient accumulation and disappearance of intermediates detected by high-pressure liquid chromatography. Inoculation of fungal-bacterial cocultures into PAH- contaminated soil resulted in significantly improved degradation of high- molecular-weight PAHs, benzo [a] pyrene mineralization (53% of added [14C] benzo [a] pyrene was recovered as 14CO2 in 100 days), and reduction in the mutagenicity of organic soil extracts, compared with the indigenous microbes and soil amended with only axenic inocula.

Item Details

Item Type:Refereed Article
Research Division:Technology
Research Group:Environmental Biotechnology
Research Field:Bioremediation
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Britz, ML (Professor Margaret Britz)
ID Code:73311
Year Published:2000
Web of Science® Times Cited:281
Deposited By:Research Division
Deposited On:2011-09-26
Last Modified:2011-09-26
Downloads:0

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