eCite Digital Repository

Evaluation of a creosote-based medium for the growth and preparation of a PAH-degrading bacterial community for bioaugmentation


Juhasz, AL and Britz, ML and Stanley, GA, Evaluation of a creosote-based medium for the growth and preparation of a PAH-degrading bacterial community for bioaugmentation, Journal of Industrial Microbiology and Biotechnology, 24, (4) pp. 277-284. ISSN 1367-5435 (2000) [Refereed Article]

DOI: doi:10.1038/sj.jim.2900819


Creosote was evaluated as an inexpensive carbon source for growing inocula of a polycyclic aromatic hydrocarbon (PAH)-degrading bacterial community (community five). Creosote was a poor growth substrate when provided as sole carbon source in a basal salts solution (BSM). Alternatively, peptone, yeast extract or glucose in BSM supported high growth rates, but community five could not subsequently degrade pyrene. A combination of creosote and yeast extract in BSM (CYEM) supported growth and maintained the pyrene-degrading capacity of community five. Optimum pyrene- degrading activity occurred when the inocula were grown in creosote and yeast extract concentrations of 2 ml L -1 and 1 g L -1 respectively: concentrations outside these values resulted in either low biomass yields or loss of PAH-degrading activity. CYEM-grown community five inocula degraded 250 mg L -1 of pyrene in BSM at a rate comparable to cultures inoculated with community five grown in BSM-pyrene. However, the CYEM-grown community showed a 40% lower rate of PAH degradation in a synthetic PAH mixture compared with pyrene-grown cells and there was an increase in the lag period before the onset of PAH degradation. This appears to reflect a weaker induction of PAH catabolism by CYEM compared to BSM-pyrene.

Item Details

Item Type:Refereed Article
Research Division:Technology
Research Group:Environmental Biotechnology
Research Field:Bioremediation
Objective Division:Environment
Objective Group:Rehabilitation of Degraded Environments
Objective Field:Rehabilitation of Degraded Environments not elsewhere classified
Author:Britz, ML (Professor Margaret Britz)
ID Code:73305
Year Published:2000
Web of Science® Times Cited:14
Deposited By:Research Division
Deposited On:2011-09-26
Last Modified:2011-09-26

Repository Staff Only: item control page