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The effect of growth-conditions of corynebacterium-glutamicum on the transformation frequency obtained by electroporation

Citation

Haynes, JA and Britz, ML, The effect of growth-conditions of corynebacterium-glutamicum on the transformation frequency obtained by electroporation, Journal of General Microbiology, 136 pp. 255-263. ISSN 0022-1287 (1990) [Refereed Article]

DOI: doi:10.1099/00221287-136-2-255

Abstract

A rapid and efficient method of transforming Corynebacterium glutamicum by electroporation is described. A number of factors are important in determining the level of transformation obtained. Cells grown in the presence of glycine and isonicotinic acid hydrazide and harvested in early exponential growth phase were much easier to transform. The recovery medium on which transformants were isolated also had a significant effect on the number of transformants obtained because cells were osmotically or electrochemically sensitive following electroporation. Transformation efficiencies of up to 5 x 10 5 transformants per μg plasmid DNA with homologously derived DNA and 2 x 10 3 transformants per μg of DNA derived from Escherichia coli were obtained.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Bacteriology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Britz, ML (Professor Margaret Britz)
ID Code:73249
Year Published:1990
Web of Science® Times Cited:46
Deposited By:Research Division
Deposited On:2011-09-22
Last Modified:2011-12-08
Downloads:0

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