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The effect of growth-conditions of corynebacterium-glutamicum on the transformation frequency obtained by electroporation
journal contribution
posted on 2023-05-17, 08:19 authored by Haynes, JA, Margaret BritzMargaret BritzA rapid and efficient method of transforming Corynebacterium glutamicum by electroporation is described. A number of factors are important in determining the level of transformation obtained. Cells grown in the presence of glycine and isonicotinic acid hydrazide and harvested in early exponential growth phase were much easier to transform. The recovery medium on which transformants were isolated also had a significant effect on the number of transformants obtained because cells were osmotically or electrochemically sensitive following electroporation. Transformation efficiencies of up to 5 x 10 5 transformants per μg plasmid DNA with homologously derived DNA and 2 x 10 3 transformants per μg of DNA derived from Escherichia coli were obtained.
History
Publication title
Journal of General MicrobiologyVolume
136Pagination
255-263ISSN
0022-1287Department/School
College Office - College of Sciences and EngineeringPublisher
Society for General MicrobiologyPlace of publication
United KingdomRepository Status
- Restricted