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Expression of chloramphenicol resistance specified by plasmid pHY416 hosted in Corynebacterium glutamicum

Citation

Britz, ML and Best, GR, Expression of chloramphenicol resistance specified by plasmid pHY416 hosted in Corynebacterium glutamicum, Current Microbiology: An International Journal, 14, (1) pp. 13-17. ISSN 0343-8651 (1986) [Refereed Article]

DOI: doi:10.1007/BF01568095

Abstract

Chloramphenicol (Cm)-resistant colonies of Corynebacterium glutamicum strain AS019 hosting plasmid pHY416 occurred at a frequency of 4×10-7 when this strain, which normally expresses kanamycin (Km) resistance only, was placed under positive selection. These isolates produced a chloramphenicol acetyltransferase (CAT) and were tenfold more resistant to Cm than the parent strain. Resistance was lost in the absence of Cm selection but could be reselected from Km-resistant clones by reapplying Cm selection. Restriction endonuclease analyses of plasmids extracted from Cm-sensitive and-resistant strains indicated that expression of CAT activity corresponded to a loss of 0.9 kb of DNA from plasmid pHY416. © 1986 Springer-Verlag New York Inc.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Bacteriology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Britz, ML (Professor Margaret Britz)
ID Code:73244
Year Published:1986
Web of Science® Times Cited:6
Deposited By:Research Division
Deposited On:2011-09-22
Last Modified:2011-09-22
Downloads:0

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