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Characterization of latent and active forms of cartilage proteinases produced by normal immature rabbit articular-cartilage in tissue-culture

Citation

Cartwright, EC and Campbell, IK and Britz, ML and Sandy, JD and Lowther, DA, Characterization of latent and active forms of cartilage proteinases produced by normal immature rabbit articular-cartilage in tissue-culture, Arthritis and Rheumatism, 26, (8) pp. 984-993. ISSN 0004-3591 (1983) [Refereed Article]

DOI: doi:10.1002/art.1780260807

Abstract

Cultured tissue slices from normal immature rabbit articular cartilage released latent neutral metal‐loproteinases into serum‐free medium. On activation with 4‐aminophenylmercuric acetate, these metalloproteinases could degrade collagen, proteoglycan, and gelatin. Also produced were an acid proteinase with the properties of cathepsin D and an inhibitor of the neutral metalloproteinases. The appearance of both the proteinases and the inhibitor in the culture medium could be prevented by incubation of cultures with cycloheximide. The active and latent forms of the proteinases were characterized using Ultrogel AcA 54 chromatography. Copyright © 1983 American College of Rheumatology

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Enzymes
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Clinical Health (Organs, Diseases and Abnormal Conditions) not elsewhere classified
Author:Britz, ML (Professor Margaret Britz)
ID Code:73240
Year Published:1983
Web of Science® Times Cited:20
Deposited By:Research Division
Deposited On:2011-09-22
Last Modified:2011-09-22
Downloads:0

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