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Isolation and properties of metronidazole-resistant mutants of clostridium-perfringens


Sindar, P and Britz, ML and Wilkinson, RG, Isolation and properties of metronidazole-resistant mutants of clostridium-perfringens, Journal of Medical Microbiology, 15, (4) pp. 503-509. ISSN 0022-2615 (1982) [Refereed Article]

DOI: doi:10.1099/00222615-15-4-503


Clostridium perfringens strains resistant to metronidazole and tinidazole were isolated from the sensitive parent strain CM288 after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. Strain CM288 was already resistant to rifampicin and nalidixic acid; these genetic markers helped to confirm the identity of mutants. All mutants showed similar characteristics: they grew more slowly than the parent strain and failed to reach the same maximum turbidity; uptake of metronidazole and tinidazole from culture fluids was slow and end products of glucose metabolism were different from those of the parent. Pyruvate dehydrogenase activity was not detected in broken cell preparations of the mutant strains although this enzyme was readily detected in the parent strain. Changes in end products of glucose metabolism were consistent with the absence of pyruvate dehydrogenase activity because pyruvate was accumulated during growth and lactate levels were higher whereas acetate, CO 2 and ethanol levels were diminished.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbiology not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Britz, ML (Professor Margaret Britz)
ID Code:73235
Year Published:1982
Web of Science® Times Cited:29
Deposited By:Research Division
Deposited On:2011-09-22
Last Modified:2011-09-22

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