University of Tasmania
Browse

File(s) not publicly available

Chloramphenicol acetyltransferase of bacteroides-fragilis

journal contribution
posted on 2023-05-17, 08:19 authored by Margaret BritzMargaret Britz, Wilkinson, RG
Chloramphenicol-resistant strains of B. fragilis (minimum inhibitory concentration, 12.5 μg/ml) were isolated from a stool specimen which contained multiply resistant Escherichia coli. The enzyme responsible for resistance, chloramphenicol acetyltransferase, was produced constitutively by these strains; the specific activity was 10-fold lower than that of the E. coli enzymes. Similar activity was not detected in susceptible B. fragilis strains, nor could it be induced by growth in the presence of chloramphenicol or by mutagenesis. The enzyme had a pH optimum of 7.8 and a molecular weight of approximately 89,000. The Km for chloramphenicol was 5.2 μM, and the enzyme was sensitive to inhibition by 5.5'-dithiobis-2-nitrobenzoic acid. The enzyme produced by an E. coli strain isolated from the same specimen had a similar Km and sensitivity to 5.5'-dithiobis-2-nitrobenzoic acid.

History

Publication title

Antimicrobial Agents and Chemotherapy

Volume

14

Pagination

105-111

ISSN

0066-4804

Department/School

College Office - College of Sciences and Engineering

Publisher

Amer Soc Microbiology

Place of publication

1752 N St Nw, Washington, USA, Dc, 20036-2904

Repository Status

  • Restricted

Socio-economic Objectives

Clinical health not elsewhere classified

Usage metrics

    University Of Tasmania

    Categories

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC