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Smoking cessation but not inhaled corticosteroids (ICS) can increase histone deacetylase 2 (HDAC2)] in COPD


Sohal, SS and Reid, D and Soltani, A and Weston, S and Muller, HK and Wood-Baker, R and Walters, EH, Smoking cessation but not inhaled corticosteroids (ICS) can increase histone deacetylase 2 (HDAC2)] in COPD , The European Respiratory Journal , pp. 785s. (2010) [Conference Extract]


Introduction: The expression of anti-inflammatory HDAC2 may be reduced in COPD1 due to increased oxidative stress secondary to airway inflammation. This may induce resistance to ICS. Objective: To confirm the extent of suppressed HDAC2 immunostaining within the airways of COPD; its reversibility with ICS or smoking cessation. Methods: Endobronchial biopsies (ebb) from current smoking with COPD (CS; n=15), ex-smokers with COPD (ES; n=17) and normal controls (NC; n=9) were immunostained for HDAC2. COPD subjects entered a double blind randomised controlled trial comparing fluticasone propionate (0.5 mg/twice daily) with placebo for 6 months; ebb were again obtained after treatment and effects of ICS on HDAC2 assessed. Results: Compared to NC there was normal epithelial HDAC2 staining but signif- icant reduction in HDAC2 positive cells in the lamina propria (LP) in CS (p=0.02). However, this was due to a reduction in total LP cells (p = 0.04) and % cell HDAC2 staining was normal. This total cell change strongly negatively correlated to smoking history (R = -0.8, p<0.003). There were significantly more HDAC2 positive cells in ES compared to CS (p=0.04), and this was due to an increase in % cell staining and not increase in total cell numbers. ICS made no difference to HDAC2 staining. Conclusions: Our finding suggests that HDAC2 expression is reduced in CS but due to confounding by changes in cellularity in LP. Quitting does seem to have a real effect on upregulating HDAC2 at a cell level, but is not affected by ICS. Prospective long term smoking cessation studies are needed to confirm these preliminary findings.

Item Details

Item Type:Conference Extract
Research Division:Biomedical and Clinical Sciences
Research Group:Cardiovascular medicine and haematology
Research Field:Respiratory diseases
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Sohal, SS (Dr Sukhwinder Sohal)
UTAS Author:Reid, D (Dr David Reid)
UTAS Author:Soltani, A (Dr Amir Soltani Abhari)
UTAS Author:Weston, S (Mr Steven Weston)
UTAS Author:Muller, HK (Professor Konrad Muller)
UTAS Author:Wood-Baker, R (Professor Richard Wood-Baker)
UTAS Author:Walters, EH (Professor Haydn Walters)
ID Code:73026
Year Published:2010
Deposited By:Menzies Institute for Medical Research
Deposited On:2011-09-07
Last Modified:2011-09-14

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