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Intestinal protective effect of a commercial fish protein hydrolysate preparation


Marchbank, T and Elia, G and Playford, RJ, Intestinal protective effect of a commercial fish protein hydrolysate preparation, Regulatory Peptides, 155, (1-3) pp. 105-109. ISSN 0167-0115 (2009) [Refereed Article]

DOI: doi:10.1016/j.regpep.2009.02.003


Objectives: A partially hydrolysed, dried, product of pacific whiting fish is marketed as a health food supplement supporting 'intestinal health'. Scientific data supporting these claims are severely limited. We, therefore, examined if it influenced intestinal injury caused by the NSAID, indomethacin. Methods: Effects of fish hydrolysate on proliferation ([3H]-thymidine) and indomethacin-induced apoptosis (active caspase-3-immunostaining) utilised HT29 cells. In vivo studies used mice (n = 8/group). 4/6 groups had fish hydrolysate (25 or 50 mg/ml) supplemented to their drinking water for 7 days. All mice received indomethacin (85 mg/kg subcutaneously) or placebo, 12 h before killing. Small intestinal injury was assessed using morphometry and morphology, proliferation (crypt BrdU labelling ) and apoptosis (active caspase-3 immunostaining). Results: Fish hydrolysate stimulated proliferation of HT29 cells. Apoptosis increased 3-fold following incubation with indomethacin but co-presence of fish hydrolysate truncated this effect by 40% (p < 0.01). In mice, fish hydrolysate reduced the villus damaging effects of indomethacin by 60% (p < 0.05). Indomethacin increased intestinal proliferation by 65%, irrespective of presence of hydrolysate. In contrast, intestinal caspase-3 activity increased by 83% in animals given indomethacin but this rise was truncated by 70% by co-presence of hydrolysate (p < 0.01). Conclusion: This natural bioactive product reduced apoptosis and the gut damaging effects of indomethacin. © 2009 Elsevier B.V. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Clinical sciences
Research Field:Gastroenterology and hepatology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Playford, RJ (Professor Ray Playford)
ID Code:72987
Year Published:2009
Web of Science® Times Cited:15
Deposited By:Research Division
Deposited On:2011-09-05
Last Modified:2011-09-05

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