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Bioactive recombinant human lactoferrin, derived from rice, stimulates mammalian cell growth


Huang, N and Bethell, D and Card, C and Cornish, J and Marchbank, T and Wyatt, D and Mabery, K and Playford, RJ, Bioactive recombinant human lactoferrin, derived from rice, stimulates mammalian cell growth, In Vitro Cellular and Developmental Biology - Animal, 44, (10) pp. 464-471. ISSN 1071-2690 (2008) [Refereed Article]

DOI: doi:10.1007/s11626-008-9136-7


Today there is a concern about the use of animal source proteins and peptides in cell culture applications due to potential contamination by adventitious infectious pathogens. Recombinant production of these proteins using a plant host provides a safe and cost effective alternative. In this paper, we tested the effect of rice-derived recombinant human lactoferrin (rhLF) on mammalian cell growth. The purified rhLF was partially (about 50%) iron-saturated (pis-rhLF). Chemical modification of pis-rhLF generated apo-rhLF (<10% iron saturation) or holo-rhLF (>90% iron saturation). All three forms of rhLF (pis, apo, holo) promoted growth of intestinal cells (HT-29) measured as [3H]-thymidine incorporation or viable cell count, but holo-rhLF was most effective. Holo-rhLF was further tested on hybridoma, osteoblast, and human embryonic kidney cells. Results showed that holo-rhLF promoted cell growth and reduced cell doubling time. The concentration of holo-rhLF in media was critical in promoting cell growth and each cell line had different concentration dependence with the most effective range from 5 to 200 mg/L. The effect of rhLF on antibody production was determined using a hybridoma cell line. Significantly, more antibodies were produced by cells grown with holo-rhLF than cells grown without holo-rhLF. We also compared the effect of holo-rhLF to that of human transferrin, a component commonly used in cell culture media as an iron source. Holo-rhLF was as effective as human transferrin in promoting cell growth and antibody production. Considering all the data obtained, we conclude that rhLF from rice is effective in promoting mammalian cell growth and increasing cell productivity. © 2008 The Society for In Vitro Biology.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Medical biotechnology
Research Field:Medical biotechnology not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the health sciences
UTAS Author:Playford, RJ (Professor Ray Playford)
ID Code:72984
Year Published:2008
Web of Science® Times Cited:33
Deposited By:Research Division
Deposited On:2011-09-05
Last Modified:2011-09-05

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