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Human spasmolytic polypeptide is a cytoprotective agent that stimulates cell migration

Citation

Playford, RJ and Marchbank, T and Chinery, R and Evison, R and Pignatelli, M and Boulton, RA and Thim, L and Hanby, AM, Human spasmolytic polypeptide is a cytoprotective agent that stimulates cell migration, Gastroenterology: A Journal Devoted to The Clinical and Basic Studies of The Digestive Tract and Liver, 108, (1) pp. 108-116. ISSN 0016-5085 (1995) [Refereed Article]

DOI: doi:10.1016/0016-5085(95)90014-4

Abstract

Background/Aims: Gastric epithelium is attacked by acid, pepsin, and ingested agents. When a mucosal lesion occurs, the defect is rapidly closed by cell migration. Because spasmolytic polypeptide is rapidly produced at sites of injury, we postulated that human spasmolytic polypeptide (hSP) was important in mucosal repair. Recombinant hSP was used to test this hypothesis. Methods: The ulcer healing effect of various doses of hSP administered orally and subcutaneously was examined using an indomethacin (20 mg/kg) restraint rat model of gastric damage. Stability of hSP in gastrointestinal juice was determined using size-exclusion chromatography. The effect of hSP on migration of human colonic carcinoma cell lines HT29 and SW480 was determined using collagen gel invasion and wounded monolayer assays. Proliferation was assessed using [3H]thymidine incorporation and toluidine blue staining. Results: Infusions of hSP at 25 and 50 μg·kg-1·h-1 subcutaneously decreased gastric damage by about 50% (P < 0.01) without changing acid secretion. Oral hSP was ineffective. hSP was stable in gastrointestinal juice. hSP stimulated migration of HT29 cells but did not affect proliferation and had no effect on SW480 cells. Conclusions: hSP may play a key role in the early stages of mucosal repair by stimulating the initial re-epithelialization by cell migration. © 1995.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Clinical Sciences
Research Field:Gastroenterology and Hepatology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Digestive System Disorders
Author:Playford, RJ (Professor Ray Playford)
ID Code:72839
Year Published:1995
Web of Science® Times Cited:254
Deposited By:Research Division
Deposited On:2011-09-01
Last Modified:2011-09-05
Downloads:0

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