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Gastric output of pancreatic secretory trypsin inhibitor is increased by misoprostol


Playford, RJ and Batten, JJ and Freeman, TC and Beardshall, K and Vesey, DA and Fenn, GC and Baron, JH and Calam, J, Gastric output of pancreatic secretory trypsin inhibitor is increased by misoprostol, Gut: An International Journal of Gastroenterology and Hepatology, 32, (11) pp. 1396-1400. ISSN 0017-5749 (1991) [Refereed Article]

DOI: doi:10.1136/gut.32.11.1396


Pancreatic secretory trypsin inhibitor (PSTI) is a potent protease inhibitor that also has growth promoting activity. It has recently been identified in the foveolar cells of the stomach, which secrete mucus. We examined the effects of the prostaglandin E1 analogue misoprostol on gastric PSTI output. Seven normal volunteers took part. An initial period ofgastric aspiration was followed by four 40 minute periods of gastric perfusion at 5 ml/minute of: 0·14 molll saline, 0·17 mmol/l bicarbonate, bicarbonate with misoprostol 400 μg, and then bicarbonate again. All perfusates contained polyethylene glycol 4000 as a marker. Misoprostol increased median gastric secretion of PSTI from 11 to 33 μg/hour (p<0·5), producing concentrations in gastric juice six times higher than those found in jejunal juice and about 1/30 of the values seen in pancreatic juice. Median mucus secretion increased to a lesser extent from 29 to 38 mg/hour during misoprostol. There was no change in intragastric concentrations of protein or of epidermal growth factor during infusion of misoprostol. Infusion of pentagastrin (6 μg/kg/ hour) had no effect on gastric secretion of mucus, PSTI, or protein. Human gastric mucus was degraded on incubation with trypsin in vitro and this was prevented by the addition of PSTI. These results suggest that gastric PSTI may protect the gastric mucus layer against refluxed pancreatic proteases. Increased output of PSTI during microprostol may contribute to the protective effect of this drug.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Clinical sciences
Research Field:Gastroenterology and hepatology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Playford, RJ (Professor Ray Playford)
ID Code:72803
Year Published:1991
Web of Science® Times Cited:25
Deposited By:Research Division
Deposited On:2011-08-31
Last Modified:2011-09-05

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