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Monolithic porous layer open tubular (monoPLOT) columns for low pressure liquid chromatography of proteins


Nesterenko, E and Yavorska, O and Macka, M and Yavorskyy, A and Paull, B, Monolithic porous layer open tubular (monoPLOT) columns for low pressure liquid chromatography of proteins, Analytical Methods, 3, (3) pp. 537-543. ISSN 1759-9660 (2011) [Refereed Article]

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Copyright Statement

Copyright 2011 Royal Society of Chemistry

DOI: doi:10.1039/c0ay00649a


Glycidyl methacrylate-ethylene dimethacrylate (GMA-co-EDMA) based monolithic porous layer open tubular (monoPLOT) columns (0.05 mm I.D., monolithic layer thickness approximate to 5 mu m) have been fabricated using an automated column scanning technique, providing UV polymerisation at 365 nm. Columns were chemically modified to obtain desired diol groups on the surface, and the longitudinal homogeneity of the stationary phase was profiled using scanning capacitively coupled contactless conductivity detector (sC(4)D), before and after such modification. Using the automated scanning polymerisation technique, column-to-column production reproducibility, including longitudinal phase thickness, was within +/-5% RSD. The prepared columns were tested to evaluate their liquid chromatographic stationary phase selectivity, efficiency and reproducibility, with a series of test protein mixtures. Under optimised gradient conditions, the separation of up to 8 proteins was demonstrated on the open tubular column (510 x 0.05 mm I.D.), with a column pressure drop of <1.5 MPa

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Macka, M (Professor Mirek Macka)
UTAS Author:Paull, B (Professor Brett Paull)
ID Code:72698
Year Published:2011
Web of Science® Times Cited:26
Deposited By:Chemistry
Deposited On:2011-08-30
Last Modified:2017-10-29

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