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A new PCR assay for reliable molecular sexing of endangered Tasmanian devils (Sarcophilus harrisii) from non-invasive genetic samples

Citation

Lachish, S and Passmore, A and Jones, M, A new PCR assay for reliable molecular sexing of endangered Tasmanian devils (Sarcophilus harrisii) from non-invasive genetic samples, Conservation Genetics Resources, 3, (2) pp. 279-281. ISSN 1877-7252 (2011) [Refereed Article]


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The final publication is available at http://www.springerlink.com

DOI: doi:10.1007/s12686-010-9341-z

Abstract

Sex identification is a critical component of threatened species monitoring programs. For cryptic, rare and endangered species that have low detection probabilities, efficient monitoring can be achieved by analysing non-invasively collected DNA with molecular genetic techniques. In this study, we report a multiplex PCR-based sexing assay for the endangered Tasmanian devil (Sarcophilus harrisii). The assay uses a new species-specific primer set that amplifies a fragment of the SRY gene and an autosomal microsatellite marker as an internal positive control. We show that this assay provides highly accurate and robust sex identification (99% accuracy [95%CI 96.50.99.8]) where sex could be assigned of devil DNA from tissue, hair and faeces. This simple, yet reliable sexing assay is an important step towards effective monitoring and management of Tasmanian devils.

Item Details

Item Type:Refereed Article
Keywords:SRY, molecular sexing,Tasmanian devil, non-invasive genetic monitoring
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Population, Ecological and Evolutionary Genetics
Objective Division:Environment
Objective Group:Flora, Fauna and Biodiversity
Objective Field:Flora, Fauna and Biodiversity at Regional or Larger Scales
Author:Lachish, S (Ms Shelly Lachish)
Author:Passmore, A (Dr Abraham Passmore)
Author:Jones, M (Associate Professor Menna Jones)
ID Code:72621
Year Published:2011
Web of Science® Times Cited:3
Deposited By:Zoology
Deposited On:2011-08-30
Last Modified:2018-03-17
Downloads:3 View Download Statistics

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