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Coronavirus-induced encephalomyelitis: an immunodominant CD4(+) T cell site on the nucleocapsid protein contributes to protection

Citation

Wege, H and Schliephake, A and Korner, H and Flory, E and Wege, H, Coronavirus-induced encephalomyelitis: an immunodominant CD4(+) T cell site on the nucleocapsid protein contributes to protection, Advances in Experimental Medicine and Biology, 342 pp. 413-418. ISSN 0065-2598 (1993) [Refereed Article]

Abstract

In this communication we present clear evidence, that the N-protein of MHV-JHM contains immunodominant CD4+ T-cell sites. These sites were recognized by the immune system of virus infected Lewis rats. In previous investigations we have shown, that CD4+ T-cell lines with specificity for defined viral proteins can be selected from diseased Lewis rats and mediate protection, if transferred to otherwise lethally infected animals. To define regions of the N-protein, which are immunodominant for the T-cell response, we employed bacterially expressed N-protein and truncated subfragments of N as an antigen. We demonstrate, that T-cells from MHV-JHM infected, diseased Lewis rats recognized with high prevalence the carboxyterminal subfragment C4-N (95 aa) and to some extent the adjacent C3-N protein. The same results were obtained with T-cells derived from rats immunized with bacterially expressed N-protein or from animals vaccinated by a stable N-protein expressing vaccinia recombinant. Finally, transfer of CD4+ line T-cells to MHV-JHM infected rats specific for C4-N mediated protection against acute disease.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Immunology not elsewhere classified
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Infectious Diseases
Author:Korner, H (Professor Heinrich Korner)
ID Code:72582
Year Published:1993
Deposited By:Research Division
Deposited On:2011-08-29
Last Modified:2012-07-02
Downloads:0

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