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Gene knock-out technology - a methodological overview for the interested novice

journal contribution
posted on 2023-05-17, 07:48 authored by Gallitaliadoros, LA, Sedgwick, JD, Wood, SA, Heinrich KornerHeinrich Korner
Gene targeting by homologous recombination is a powerful technique, generating mouse strains with defined mutations in their genome. These genetically modified, 'designer' animals allow us for the first time to ask simple questions about elaborate and complex biological systems. Dissecting the function of individual components of the immune system is a perfect application of this technology. Although the techniques involved in the generation of gene knock-out mice are increasingly well defined, to many immunologists the language and concepts are confusing. This review presents the essentials of the technology in a form digestible by the non-expert. © 1995.

History

Publication title

Journal of Immunological Methods

Volume

181

Pagination

1-15

ISSN

0022-1759

Department/School

Menzies Institute for Medical Research

Publisher

Elsevier Science Bv

Place of publication

Po Box 211, Amsterdam, Netherlands, 1000 Ae

Repository Status

  • Restricted

Socio-economic Objectives

Clinical health not elsewhere classified

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