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Gene knock-out technology - a methodological overview for the interested novice

Citation

Gallitaliadoros, LA and Sedgwick, JD and Wood, SA and Korner, H, Gene knock-out technology - a methodological overview for the interested novice , Journal of Immunological Methods, 181, (1) pp. 1-15. ISSN 0022-1759 (1995) [Refereed Article]

DOI: doi:10.1016/0022-1759(95)00017-5

Abstract

Gene targeting by homologous recombination is a powerful technique, generating mouse strains with defined mutations in their genome. These genetically modified, 'designer' animals allow us for the first time to ask simple questions about elaborate and complex biological systems. Dissecting the function of individual components of the immune system is a perfect application of this technology. Although the techniques involved in the generation of gene knock-out mice are increasingly well defined, to many immunologists the language and concepts are confusing. This review presents the essentials of the technology in a form digestible by the non-expert. © 1995.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Immunology not elsewhere classified
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Infectious Diseases
Author:Korner, H (Professor Heinrich Korner)
ID Code:72567
Year Published:1995
Web of Science® Times Cited:54
Deposited By:Research Division
Deposited On:2011-08-29
Last Modified:2011-08-29
Downloads:0

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