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Gene knock-out technology - a methodological overview for the interested novice
journal contribution
posted on 2023-05-17, 07:48 authored by Gallitaliadoros, LA, Sedgwick, JD, Wood, SA, Heinrich KornerHeinrich KornerGene targeting by homologous recombination is a powerful technique, generating mouse strains with defined mutations in their genome. These genetically modified, 'designer' animals allow us for the first time to ask simple questions about elaborate and complex biological systems. Dissecting the function of individual components of the immune system is a perfect application of this technology. Although the techniques involved in the generation of gene knock-out mice are increasingly well defined, to many immunologists the language and concepts are confusing. This review presents the essentials of the technology in a form digestible by the non-expert. © 1995.
History
Publication title
Journal of Immunological MethodsVolume
181Pagination
1-15ISSN
0022-1759Department/School
Menzies Institute for Medical ResearchPublisher
Elsevier Science BvPlace of publication
Po Box 211, Amsterdam, Netherlands, 1000 AeRepository Status
- Restricted