CD8 alpha- and Langerin-negative dendritic cells, but not Langerhans cells, act as principal antigen-presenting cells in leishmaniasis
You are here
Ritter, U and Meissner, A and Scheidig, C and Korner, H, CD8 alpha- and Langerin-negative dendritic cells, but not Langerhans cells, act as principal antigen-presenting cells in leishmaniasis, European Journal of Immunology, 34, (6) pp. 1542-1550. ISSN 0014-2980 (2004) [Refereed Article]
In the early phase of leishmaniasis three types of potential antigen-presenting cells, including epidermal Langerhans cells (LC), dermal dendritic cells (DC) and inflammatory DC, are localized at the site of infection. Therefore, it has been a central question which cell type is responsible for the initiation of a protective immune response. In the early stage of an anti-Leishmania immune response, detectable Leishmania major antigen was localized in the paracortex of the draining lymph nodes (LN). Characterization of antigen-positive cells showed that L. major co-localized with DC of a CD11c+ CD8α - Langerin- phenotype. To determine the area of antigen uptake, dermis or epidermis, and to further define the type of antigen -transporting cells, L. major was inoculated subcutaneously and concurrently LC were mobilized with fluorescein isothiocyanate (FITC . After 3 days, DC carrying L. major antigen were always FITC-, indicating a dermal and not an epidermal origin. Moreover, addition of L. major antigen to ex vivo isolated CD8α - and CD8α+ DC from the draining LN of L. major-infected C57BL/6 mice demonstrated that both DC subpopulations were able to stimulate antigen-specific T cell proliferation in vitro. Without addition of exogenous antigen only the CD8α- Langerin- DC were capable of stimulating antigen-specific T cell proliferation. Thus, we demonstrate that CD8α- Langerin DC and not LC are the basis of the protective immune response to intracellular L. major parasites in vivo. © 2004 Wiley-VCH Verlag GmbH & Co. KGaA.
Repository Staff Only:
item control page