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CC chemokine ligand 20 partially controls adhesion of naive B cells to activated endothelial cells under shear stress

Citation

Meissner, A and Zilles, O and Varona, R and Jozefowski, K and Ritter, U and Marquez, G and Hallmann, R and Korner, H, CC chemokine ligand 20 partially controls adhesion of naive B cells to activated endothelial cells under shear stress, Blood, 102, (8) pp. 2724-2727. ISSN 0006-4971 (2003) [Refereed Article]

DOI: doi:10.1182/blood-2003-01-0007

Abstract

Chemokines are thought to control lymphocyte recruitment to the inflamed endothelium. To dissect chemokine-mediated adhesion, binding of ex vivo isolated splenocytes to tumor necrosis factor (TNF)-activated endothelial cells was analyzed under shear stress. We observed specific adhesion of naive follicular B cells, which could be blocked by pertussis toxin. This indicated a G protein-mediated binding and pointed at a contribution of chemokine receptors to B-cell adhesion. Analysis of chemokines expressed by TNF-activated endothelial cells showed that CC chemokine ligand 2 (CCL2), CCL17, and CCL20 were up-regulated. Only on follicular B cells was the cognate receptor for CCL20, CC chemokine receptor 6 (CCR6), expressed strongly, and a functional transmigration assay with CCR6-negative B cells demonstrated conclusively the sole signaling of CCL20 through CCR6. Desensitization of CCR6 on naive B cells with CCL20 resulted in receptor down-regulation and reduced B-cell adhesion. We conclude that CCL20 plays a vital role in B-cell adhesion to the inflamed endothelium. © 2003 by The American Society of Hematology.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Immunology not elsewhere classified
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Infectious Diseases
Author:Korner, H (Professor Heinrich Korner)
ID Code:72475
Year Published:2003
Web of Science® Times Cited:25
Deposited By:Research Division
Deposited On:2011-08-26
Last Modified:2011-09-27
Downloads:0

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