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CC chemokine ligand 20 partially controls adhesion of naive B cells to activated endothelial cells under shear stress
journal contribution
posted on 2023-05-17, 07:46 authored by Meissner, A, Zilles, O, Varona, R, Jozefowski, K, Ritter, U, Marquez, G, Hallmann, R, Heinrich KornerHeinrich KornerChemokines are thought to control lymphocyte recruitment to the inflamed endothelium. To dissect chemokine-mediated adhesion, binding of ex vivo isolated splenocytes to tumor necrosis factor (TNF)-activated endothelial cells was analyzed under shear stress. We observed specific adhesion of naive follicular B cells, which could be blocked by pertussis toxin. This indicated a G protein-mediated binding and pointed at a contribution of chemokine receptors to B-cell adhesion. Analysis of chemokines expressed by TNF-activated endothelial cells showed that CC chemokine ligand 2 (CCL2), CCL17, and CCL20 were up-regulated. Only on follicular B cells was the cognate receptor for CCL20, CC chemokine receptor 6 (CCR6), expressed strongly, and a functional transmigration assay with CCR6-negative B cells demonstrated conclusively the sole signaling of CCL20 through CCR6. Desensitization of CCR6 on naive B cells with CCL20 resulted in receptor down-regulation and reduced B-cell adhesion. We conclude that CCL20 plays a vital role in B-cell adhesion to the inflamed endothelium. © 2003 by The American Society of Hematology.
History
Publication title
BloodVolume
102Issue
8Pagination
2724-2727ISSN
0006-4971Department/School
Menzies Institute for Medical ResearchPublisher
Amer Soc HematologyPlace of publication
1900 M Street. Nw Suite 200, Washington, USA, Dc, 20036Repository Status
- Restricted