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Migration of Salmonella typhimurioum-harboring bone marrow-derived dendritic cells towards the chemokines CCL19 and CCL21

Citation

Cheminay, C and Schoen, M and Hensel, M and Wandersee-Steinhauser, A and Ritter, U and Korner, H and Rollinghoff, M and Hein, J, Migration of Salmonella typhimurioum-harboring bone marrow-derived dendritic cells towards the chemokines CCL19 and CCL21, Microbial Pathogenesis , 32, (5) pp. 207-218. ISSN 0882-4010 (2002) [Refereed Article]

DOI: doi:10.1006/mpat.2002.0497

Abstract

Macrophages are considered as main cellular target encountered by the facultative intracellular bacterium Salmonella typhimurium. However, in orally infected mice these pathogens are first internalized by dendritic cells (DCs) that are located in the subepithelial dome of Peyer's patches. Moreover, DCs can penetrate the intestinal epithelium to sample bacteria. Here, we examined the interaction of Salmonella with bone marrow-derived DCs (BM-DCs). In order to study the role of DCs as vehicles for the dissemination of Salmonella, an in vitro model was established. In this model, Salmonella-activated BM-DCs enhanced surface expression of MHC class II and costimulatory molecules. We found that, upon maturation, BM-DCs upregulated chemokine receptor 7 (CCR7) mRNA and surface molecule expression. Salmonella-exposed DCs as well as mature DCs, but not immature DCs, were recruited towards the CC chemokines CCL19 and CCL21, two ligands of CCR7. The maturation process of DCs did neither require bacterial internalization nor viability. About one third of the migrated BM-DCs harbored intracellular bacteria, whereas the remaining two third did not contain bacteria. Salmonella, but not an apathogenic E. coli laboratory strain was capable to survive within BM-DCs. Taken together, our data implicate that DCs are first activated and subsequently utilized as carriers by Salmonella. © 2002 Published by Elsevier Science Ltd.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Immunology not elsewhere classified
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Infectious Diseases
Author:Korner, H (Professor Heinrich Korner)
ID Code:72470
Year Published:2002
Web of Science® Times Cited:26
Deposited By:Research Division
Deposited On:2011-08-26
Last Modified:2011-08-29
Downloads:0

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