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Mechanism of Cytotoxicity and Cellular Uptake of Lipophilic Inert Dinuclear Polypyridylruthenium(II) Complexes
journal contribution
posted on 2023-05-17, 07:45 authored by Pisani, MJ, Fromm, PD, Mulyana, Y, Clarke, RJ, Heinrich KornerHeinrich Korner, Heimann, K, Collins, JG, Keene, FRThe accumulation, uptake mechanism, cytotoxicity, cellular localisation of—and mode of cell death induced by—dinuclear ruthenium(II) complexes DD/LL-[{Ru(phen)2}2{m-bbn}]4+ (Rubbn), where phen is 1,10-phenanthroline, bbn is bis[4(4’-methyl-2,2’- bipyridyl)]-1,n-alkane (n=2, 5, 7, 10, 12 or 16), and the corresponding mononuclear complexes containing the bbn ligands, were studied in L1210 murine leukaemia cells. Cytotoxicity increased with linker chain length, and the DD-Rubb16 complex displayed the highest cytotoxicity of the series, with an IC50 value of 5 mm, similar to that of carboplatin in the L1210 murine leukaemia cell line. Confocal microscopy and flow cytometry studies indicated that the complexes accumulate in the mitochondria of L1210 cells, with the magnitude of cellular uptake and accumulation increasing with linking chain length in the bbn bridge of the metal complex. DD-Rubb16 entered the L1210 cells by passive diffusion (with a minor contribution from protein-mediated active transport), inducing cell death via apoptosis. Additionally, metal-complex uptake in leukaemia cells was approximately 16-times that observed in healthy B cells highlighting that the bbn series of complexes may have potential as selective anticancer drugs.
History
Publication title
ChemMedChem: chemistry enabling drug discoveryVolume
6Issue
5Pagination
848-858ISSN
1860-7179Department/School
Menzies Institute for Medical ResearchPublisher
Wiley - V C H Verlag GmbH & Co. KGaAPlace of publication
GermanyRights statement
Copyright © 2011 The definitive published version is available online at: http://onlinelibrary.wiley.com/Repository Status
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