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Mechanism of Cytotoxicity and Cellular Uptake of Lipophilic Inert Dinuclear Polypyridylruthenium(II) Complexes

Citation

Pisani, MJ and Fromm, PD and Mulyana, Y and Clarke, RJ and Korner, H and Heimann, K and Collins, JG and Keene, FR, Mechanism of Cytotoxicity and Cellular Uptake of Lipophilic Inert Dinuclear Polypyridylruthenium(II) Complexes, ChemMedChem: chemistry enabling drug discovery, 6, (5) pp. 848-858. ISSN 1860-7179 (2011) [Refereed Article]


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Copyright © 2011 The definitive published version is available online at: http://onlinelibrary.wiley.com/

DOI: doi:10.1002/cmdc.201100053

Abstract

The accumulation, uptake mechanism, cytotoxicity, cellular localisation of—and mode of cell death induced by—dinuclear ruthenium(II) complexes DD/LL-[{Ru(phen)2}2{m-bbn}]4+ (Rubbn), where phen is 1,10-phenanthroline, bbn is bis[4(4’-methyl-2,2’- bipyridyl)]-1,n-alkane (n=2, 5, 7, 10, 12 or 16), and the corresponding mononuclear complexes containing the bbn ligands, were studied in L1210 murine leukaemia cells. Cytotoxicity increased with linker chain length, and the DD-Rubb16 complex displayed the highest cytotoxicity of the series, with an IC50 value of 5 mm, similar to that of carboplatin in the L1210 murine leukaemia cell line. Confocal microscopy and flow cytometry studies indicated that the complexes accumulate in the mitochondria of L1210 cells, with the magnitude of cellular uptake and accumulation increasing with linking chain length in the bbn bridge of the metal complex. DD-Rubb16 entered the L1210 cells by passive diffusion (with a minor contribution from protein-mediated active transport), inducing cell death via apoptosis. Additionally, metal-complex uptake in leukaemia cells was approximately 16-times that observed in healthy B cells highlighting that the bbn series of complexes may have potential as selective anticancer drugs.

Item Details

Item Type:Refereed Article
Keywords:apoptosis, bioinorganic chemistry, cellular uptake, cytotoxicity, ruthenium polypyridyl complexes
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Immunology not elsewhere classified
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Infectious Diseases
Author:Korner, H (Professor Heinrich Korner)
ID Code:72466
Year Published:2011
Web of Science® Times Cited:48
Deposited By:Menzies Institute for Medical Research
Deposited On:2011-08-26
Last Modified:2012-03-26
Downloads:0

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