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A cautionary note when using pepsin as a probe for the formation of a collagen triple helix
journal contribution
posted on 2023-05-17, 07:43 authored by Bulleid, NJ, Richard WilsonRichard Wilson, Lad, UThe ability of the collagen triple helix to resist digestion with proteases has been used as a conformational probe to ascertain whether a collagenous molecule has formed a correctly aligned helix during biosynthesis or during refolding of the protein in vitro. During our studies into the synthesis and folding of a variety of engineered procollagen polypeptide chains, we noted that resistance to digestion with proteases, in particular pepsin, could be misleading and does not necessarily indicate the formation of a collagen triple helix. These results clearly show that resistance to pepsin digestion alone should not be used to indicate correct folding and that preferably an alternative assay should be used to unequivocally demonstrate the formation of a correctly aligned triple helix.
History
Publication title
Matrix Biology: Clinical and ExperimentalVolume
17Pagination
233-236ISSN
0945-053XPublisher
Elsevier Science BvPlace of publication
Po Box 211, Amsterdam, Netherlands, 1000 AeRepository Status
- Restricted