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A cautionary note when using pepsin as a probe for the formation of a collagen triple helix


Bulleid, NJ and Wilson, RR and Lad, U, A cautionary note when using pepsin as a probe for the formation of a collagen triple helix, Matrix Biology: Clinical and Experimental, 17, (3) pp. 233-236. ISSN 0945-053X (1998) [Refereed Article]

DOI: doi:10.1016/S0945-053X(98)90062-2


The ability of the collagen triple helix to resist digestion with proteases has been used as a conformational probe to ascertain whether a collagenous molecule has formed a correctly aligned helix during biosynthesis or during refolding of the protein in vitro. During our studies into the synthesis and folding of a variety of engineered procollagen polypeptide chains, we noted that resistance to digestion with proteases, in particular pepsin, could be misleading and does not necessarily indicate the formation of a collagen triple helix. These results clearly show that resistance to pepsin digestion alone should not be used to indicate correct folding and that preferably an alternative assay should be used to unequivocally demonstrate the formation of a correctly aligned triple helix.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and cell biology
Research Field:Protein trafficking
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the health sciences
UTAS Author:Wilson, RR (Dr Richard Wilson)
ID Code:72431
Year Published:1998
Web of Science® Times Cited:2
Deposited By:Central Science Laboratory
Deposited On:2011-08-26
Last Modified:2011-08-26

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