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An immunodominant CD4+ T cell site on the nucleocapsid protein of murine coronavirus contributes to protection against encephalomyelitis


Wege, H and Schliephake, A and Korner, H and Flory, E and Wege, H, An immunodominant CD4+ T cell site on the nucleocapsid protein of murine coronavirus contributes to protection against encephalomyelitis, Journal of General Virology, 74 pp. 1287-1294 . ISSN 0022-1317 (1993) [Refereed Article]

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Copyright 1993 SGM

DOI: doi:10.1099/0022-1317-74-7-1287


The murine coronavirus neurotropic strain JHM (MHV-JHM) nucleocapsid (N) protein induces a strong T-helper cell response in Lewis rats. It has been shown previously that N-specific CD4+ T cells can confer protection against acute disease upon transfer to otherwise lethally infected rats. To define the major antigenic regions that elicit this T cell response, truncated fragments of N protein were expressed from a bacterial expression vector and employed as T cell antigens. Lymphocytes from either MHV-JHM-infected or immunized rats were stimulated in culture with virus antigen, grown and tested for their specificity to the N protein fragments. The carboxy-terminally located C4-N fragment (95 amino acids) induced the most pronounced proliferative response irrespective of whether the lymphocyte culture was derived from immunized or MHV-JHM-infected rats. We established T cell lines specific for the truncated N protein fragments and tested their potential to mediate protection by transfer experiments. Only the T cell line C4-N and the T cell line specific for the full-length N protein were protective. By contrast, all truncated N protein fragments elicited a humoral immune response and contained antigenic sites recognized by antibodies from diseased rats.

Item Details

Item Type:Refereed Article
Keywords:murine coronavirus neurotropic strain, T-helper cell, CD4+ T cell, bacterial expression vector, virus antigen, lymphocyte culture, humoral immune response, antibodies, demyelination, encephalomyelitis
Research Division:Biomedical and Clinical Sciences
Research Group:Immunology
Research Field:Immunology not elsewhere classified
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Korner, H (Professor Heinrich Korner)
ID Code:72419
Year Published:1993
Web of Science® Times Cited:19
Deposited By:Research Division
Deposited On:2011-08-26
Last Modified:2012-10-08

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