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An immunodominant CD4+ T cell site on the nucleocapsid protein of murine coronavirus contributes to protection against encephalomyelitis
Citation
Wege, H and Schliephake, A and Korner, H and Flory, E and Wege, H, An immunodominant CD4+ T cell site on the nucleocapsid protein of murine coronavirus contributes to protection against encephalomyelitis, Journal of General Virology, 74 pp. 1287-1294 . ISSN 0022-1317 (1993) [Refereed Article]
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Copyright Statement
Copyright 1993 SGM
DOI: doi:10.1099/0022-1317-74-7-1287
Abstract
The murine coronavirus neurotropic strain JHM (MHV-JHM)
nucleocapsid (N) protein induces a strong T-helper
cell response in Lewis rats. It has been shown
previously that N-specific CD4+ T cells can confer
protection against acute disease upon transfer to
otherwise lethally infected rats. To define the major
antigenic regions that elicit this T cell response,
truncated fragments of N protein were expressed from a
bacterial expression vector and employed as T cell
antigens. Lymphocytes from either MHV-JHM-infected
or immunized rats were stimulated in culture with virus
antigen, grown and tested for their specificity to the N
protein fragments. The carboxy-terminally located C4-N
fragment (95 amino acids) induced the most pronounced
proliferative response irrespective of whether the lymphocyte
culture was derived from immunized or MHV-JHM-infected rats. We established T cell lines specific
for the truncated N protein fragments and tested their
potential to mediate protection by transfer experiments.
Only the T cell line C4-N and the T cell line specific for
the full-length N protein were protective. By contrast, all
truncated N protein fragments elicited a humoral
immune response and contained antigenic sites recognized
by antibodies from diseased rats.
Item Details
Item Type: | Refereed Article |
---|---|
Keywords: | murine coronavirus neurotropic strain, T-helper cell, CD4+ T cell, bacterial expression vector, virus antigen, lymphocyte culture, humoral immune response, antibodies, demyelination, encephalomyelitis |
Research Division: | Medical and Health Sciences |
Research Group: | Immunology |
Research Field: | Immunology not elsewhere classified |
Objective Division: | Health |
Objective Group: | Clinical Health (Organs, Diseases and Abnormal Conditions) |
Objective Field: | Infectious Diseases |
UTAS Author: | Korner, H (Professor Heinrich Korner) |
ID Code: | 72419 |
Year Published: | 1993 |
Web of Science® Times Cited: | 19 |
Deposited By: | Research Division |
Deposited On: | 2011-08-26 |
Last Modified: | 2012-10-08 |
Downloads: | 0 |
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