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WARP is a novel multimeric component of the chondrocyte pericellular matrix that interacts with perlecan

Citation

Allen, JM and Bateman, JF and Hansen, U and Wilson, RR and Bruckner, P and Owens, RT and Sasaki, T and Timpl, R and Fitzgerald, J, WARP is a novel multimeric component of the chondrocyte pericellular matrix that interacts with perlecan, Journal of Biological Chemistry, 281, (11) pp. 7341-7349. ISSN 0021-9258 (2006) [Refereed Article]

DOI: doi:10.1074/jbc.M513746200

Abstract

WARP is a novel member of the von Willebrand factor A domain superfamily of extracellular matrix proteins that is expressed by chondrocytes. WARP is restricted to the presumptive articular cartilage zone prior to joint cavitation and to the articular cartilage and fibrocartilaginous elements in the joint, spine, and sternum during mouse embryonic development. In mature articular cartilage, WARP is highly specific for the chondrocyte pericellular microenvironment and co-localizes with perlecan, a prominent component of the chondrocyte pericellular region. WARP is present in the guanidine-soluble fraction of cartilage matrix extracts as a disulfide-bonded multimer, indicating that WARP is a strongly interacting component of the cartilage matrix. To investigate how WARP is integrated with the pericellular environment, we studied WARP binding to mouse perlecan using solid phase and surface plasmon resonance analysis. WARP interacts with domain III-2 of the perlecan core protein and the heparan sulfate chains of the perlecan domain I with K(D) values in the low nanomolar range. We conclude that WARP forms macromolecular structures that interact with perlecan to contribute to the assembly and/or maintenance of "permanent" cartilage structures during development and in mature cartilages.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Clinical Sciences
Research Field:Orthopaedics
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Medical and Health Sciences
Author:Wilson, RR (Dr Richard Wilson)
ID Code:72413
Year Published:2006
Web of Science® Times Cited:23
Deposited By:Central Science Laboratory
Deposited On:2011-08-26
Last Modified:2011-08-26
Downloads:0

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