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Proteomic analysis of mouse growth plate cartilage
Citation
Belluoccio, D and Wilson, R and Thornton, DJ and Wallis, TP and Gorman, JJ and Bateman, JF, Proteomic analysis of mouse growth plate cartilage, Proteomics, 6, (24) pp. 6549-6553. ISSN 1615-9853 (2006) [Refereed Article]
DOI: doi:10.1002/pmic.200600191
Abstract
Cartilage is a highly specialized load-bearing tissue with a small number of cells and a high proportion of extracellular matrix (ECM). The abundance of heavily sulfated proteoglycans and a poorly soluble collagenous ECM presents a major technical challenge to 2-DE. Here we report proteomic analysis of mouse growth plate cartilage using novel methodology for tissue dissection and sample prefractionation. We have successfully resolved cartilage tissue extracts by 2-DE for the first time and identified cartilage ECM proteins by Western blotting and MS/MS.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Biomedical and Clinical Sciences |
| Research Group: | Clinical sciences |
| Research Field: | Rheumatology and arthritis |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding knowledge |
| Objective Field: | Expanding knowledge in the health sciences |
| UTAS Author: | Wilson, R (Dr Richard Wilson) |
| ID Code: | 72412 |
| Year Published: | 2006 |
| Web of Science® Times Cited: | 30 |
| Deposited By: | Central Science Laboratory |
| Deposited On: | 2011-08-26 |
| Last Modified: | 2021-02-18 |
| Downloads: | 0 |
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