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Proteomic analysis of mouse growth plate cartilage

Citation

Belluoccio, D and Wilson, RR and Thornton, DJ and Wallis, TP and Gorman, JJ and Bateman, JF, Proteomic analysis of mouse growth plate cartilage, Proteomics, 6, (24) pp. 6549-6553. ISSN 1615-9853 (2006) [Refereed Article]

DOI: doi:10.1002/pmic.200600191

Abstract

Cartilage is a highly specialized load-bearing tissue with a small number of cells and a high proportion of extracellular matrix (ECM). The abundance of heavily sulfated proteoglycans and a poorly soluble collagenous ECM presents a major technical challenge to 2-DE. Here we report proteomic analysis of mouse growth plate cartilage using novel methodology for tissue dissection and sample prefractionation. We have successfully resolved cartilage tissue extracts by 2-DE for the first time and identified cartilage ECM proteins by Western blotting and MS/MS.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Clinical Sciences
Research Field:Rheumatology and Arthritis
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Medical and Health Sciences
Author:Wilson, RR (Dr Richard Wilson)
ID Code:72412
Year Published:2006
Web of Science® Times Cited:30
Deposited By:Central Science Laboratory
Deposited On:2011-08-26
Last Modified:2011-08-26
Downloads:0

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