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Proteomic analysis of mouse growth plate cartilage
journal contribution
posted on 2023-05-17, 07:42 authored by Belluoccio, D, Richard WilsonRichard Wilson, Thornton, DJ, Wallis, TP, Gorman, JJ, Bateman, JFCartilage is a highly specialized load-bearing tissue with a small number of cells and a high proportion of extracellular matrix (ECM). The abundance of heavily sulfated proteoglycans and a poorly soluble collagenous ECM presents a major technical challenge to 2-DE. Here we report proteomic analysis of mouse growth plate cartilage using novel methodology for tissue dissection and sample prefractionation. We have successfully resolved cartilage tissue extracts by 2-DE for the first time and identified cartilage ECM proteins by Western blotting and MS/MS.
History
Publication title
ProteomicsVolume
6Issue
24Pagination
6549-6553ISSN
1615-9853Publisher
Wiley-V C H Verlag GmbhPlace of publication
Po Box 10 11 61, Weinheim, Germany, D-69451Repository Status
- Restricted