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A rapid quantitative determination of phenolic acids in Brassica oleracea by capillary zone electrophoresis

Citation

Lee, ISL and Boyce, MC and Breadmore, MC, A rapid quantitative determination of phenolic acids in Brassica oleracea by capillary zone electrophoresis, Food Chemistry, 127, (2) pp. 797-801. ISSN 0308-8146 (2011) [Refereed Article]


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DOI: doi:10.1016/j.foodchem.2011.01.015

Abstract

A simple and rapid capillary zone electrophoresis method to quantitatively determine the phenolic acid contents in brassica vegetables is described. Phenolic compounds were extracted from broccoli, broccolini, Brussels sprouts, cabbage and cauliflower and the main hydroxycinnamic acids (sinapic, ferulic, p-coumaric and caffeic acids) were isolated by solid phase extraction with C18 cartridges. Using an optimised method, the four analytes were separated in less than 7 min in a 50 mu m x 60 cm capillary with a 15 mM borate buffer (pH = 9.13) and a separation voltage of 30 kV at 30 degrees C. A linear relationship was observed for the method (r = 0.9997-0.9999) with detection limits ranging from 1.1 to 2.3 mg/kg of vegetables for the analytes. This method demonstrated good reproducibility with coefficients of variation of less than 5% for peak area and less than 1% for migration time (n = 7). The method was successfully applied to quantitatively determine the phenolic acid contents in a range of brassica vegetables and the results were in good agreement when compared to those from high performance liquid chromatography analysis. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Breadmore, MC (Professor Michael Breadmore)
ID Code:72265
Year Published:2011
Funding Support:Australian Research Council (DP0984745)
Web of Science® Times Cited:37
Deposited By:Chemistry
Deposited On:2011-08-24
Last Modified:2015-02-05
Downloads:2 View Download Statistics

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