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This HPLC tutorial focuses on the preparation and use of kinetic plots to characterise the performance in isocratic and gradient LC. This graphical approach allows the selection of columns (i.e. optimum particle size and column length) and LC conditions (operating pressure and temperature) to generate a specific number of plates or peak capacity in the shortest possible analysis time. Instrument aspects including the influence of extracolumn effects (maximum allowable system volume) and thermal operating conditions (oven type) on performance are discussed. In addition, the performance characteristics of porous-shell particle-packed columns and monolithic stationary phases are presented and the potential of future column designs is discussed.

Item Type:	Refereed Article
Research Division:	Chemical Sciences
Research Group:	Analytical chemistry
Research Field:	Separation science
Objective Division:	Expanding Knowledge
Objective Group:	Expanding knowledge
Objective Field:	Expanding knowledge in the chemical sciences
UTAS Author:	Causon, TJ (Mr Timothy Causon)
UTAS Author:	Hilder, EF (Professor Emily Hilder)
UTAS Author:	Shellie, RA (Professor Robert Shellie)
ID Code:	72251
Year Published:	2011
Funding Support:	Australian Research Council (DP0987318)
Web of Science® Times Cited:	22
Deposited By:	Chemistry
Deposited On:	2011-08-24
Last Modified:	2012-05-10
Downloads:	2 View Download Statistics