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Release of extracellular purines from plant roots and effect on ion fluxes


Dark, A and Demidchik, V and Richards, SL and Shabala, S and Davies, JM, Release of extracellular purines from plant roots and effect on ion fluxes, Plant Signalling & Behavior, 6, (11) pp. 1855-1857. ISSN 1559-2316 (2012) [Refereed Article]

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Copyright 2011 Landes Biosciences

DOI: doi:10.4161/psb.6.11.17014


Extracellular purine nucleotides appear capable of regulating plant development, defense and stress responses by acting in part as agonists of plasma membrane calcium channels. Factors stimulating ATP release include wounding, osmotic stress and elicitors. Here we show that exogenous abscisic acid and L-glutamate can also cause ATP accumulation around Arabidopsis thaliana roots. Release of ADP from root epidermis would trigger ionotropic receptor-like activity in the plasma membrane, resulting in transient elevation of cytosolic free calcium. Root epidermal protoplasts (expressing aequorin as a cytosolic free calcium reporter) can support an extracellular ADP-induced cytosolic calcium elevation in the presence of an extracellular reductant. This confirms that ADP could elicit calcium-based responses distinct to those of ATP, which have been shown previously to involve production of extracellular reactive oxygen species.

Item Details

Item Type:Refereed Article
Keywords:ADP, ATP, abscisic acid, calcium, channel, glutamate, root
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Other plant production and plant primary products
Objective Field:Other plant production and plant primary products not elsewhere classified
UTAS Author:Shabala, S (Professor Sergey Shabala)
ID Code:72238
Year Published:2012
Deposited By:Agricultural Science
Deposited On:2011-08-24
Last Modified:2013-06-12

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