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Receptor-like activity evoked by extracellular ADP in Arabidopsis root epidermal plasma membrane

journal contribution
posted on 2023-05-17, 07:30 authored by Demidchik, V, Shang, Z, Shin, R, Colaco, R, Laohavisit, A, Sergey ShabalaSergey Shabala, Davies, JM
Extracellular purine nucleotides are implicated in the control of plant development and stress responses. While extracellular ATP is known to activate transcriptional pathways via plasma membrane (PM) NADPH oxidase and calcium channel activation, very little is known about signal transduction by extracellular ADP. Here, extracellular ADP was found to activate net Ca2+ influx in roots of Arabidopsis (Arabidopsis thaliana) and transiently elevate cytosolic free Ca2+ in root epidermal protoplasts. An inward Ca2+-permeable conductance in root epidermal PM was activated within 1 s of ADP application and repeated application evoked a smaller current. Such response speed and densitization are consistent with operation of equivalents to animal ionotropic purine receptors, although to date no equivalent genes for such receptors have been identified in higher plants. In contrast to ATP, extracellular ADP did not evoke accumulation of intracellular reactive oxygen species. While high concentrations of ATP caused net Ca2+ efflux from roots, equivalent concentrations of ADP caused net influx. Overall the results point to a discrete ADP signaling pathway, reliant on receptor-like activity at the PM.

History

Publication title

Plant Physiology

Volume

156

Pagination

1375-1385

ISSN

0032-0889

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

American Society of Plant Biologists

Place of publication

15501 Monona Drive, Rockville, USA, Md, 20855

Rights statement

© 2011 American Society of Plant Biologists

Repository Status

  • Restricted

Socio-economic Objectives

Other plant production and plant primary products not elsewhere classified

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