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Receptor-like activity evoked by extracellular ADP in Arabidopsis root epidermal plasma membrane

Citation

Demidchik, V and Shang, Z and Shin, R and Colaco, R and Laohavisit, A and Shabala, S and Davies, JM, Receptor-like activity evoked by extracellular ADP in Arabidopsis root epidermal plasma membrane, Plant Physiology, 156, (3) pp. 1375-1385. ISSN 0032-0889 (2011) [Refereed Article]


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Copyright Statement

2011 American Society of Plant Biologists

DOI: doi:10.1104/pp.111.174722

Abstract

Extracellular purine nucleotides are implicated in the control of plant development and stress responses. While extracellular ATP is known to activate transcriptional pathways via plasma membrane (PM) NADPH oxidase and calcium channel activation, very little is known about signal transduction by extracellular ADP. Here, extracellular ADP was found to activate net Ca2+ influx in roots of Arabidopsis (Arabidopsis thaliana) and transiently elevate cytosolic free Ca2+ in root epidermal protoplasts. An inward Ca2+-permeable conductance in root epidermal PM was activated within 1 s of ADP application and repeated application evoked a smaller current. Such response speed and densitization are consistent with operation of equivalents to animal ionotropic purine receptors, although to date no equivalent genes for such receptors have been identified in higher plants. In contrast to ATP, extracellular ADP did not evoke accumulation of intracellular reactive oxygen species. While high concentrations of ATP caused net Ca2+ efflux from roots, equivalent concentrations of ADP caused net influx. Overall the results point to a discrete ADP signaling pathway, reliant on receptor-like activity at the PM.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Other Plant Production and Plant Primary Products
Objective Field:Plant Production and Plant Primary Products not elsewhere classified
Author:Shabala, S (Professor Sergey Shabala)
ID Code:72222
Year Published:2011
Web of Science® Times Cited:28
Deposited By:Agricultural Science
Deposited On:2011-08-24
Last Modified:2017-11-02
Downloads:0

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