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Retention behavior and selectivity of a latex nanoparticle pseudostationary phase for electrokinetic chromatography


Palmer, CP and Keeffer, A and Hilder, EF and Haddad, PR, Retention behavior and selectivity of a latex nanoparticle pseudostationary phase for electrokinetic chromatography, Electrophoresis, 32, (5) pp. 588-594. ISSN 0173-0835 (2011) [Refereed Article]

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DOI: doi:10.1002/elps.201000470


The retention characteristics and separation selectivity of a novel latex nanoparticle (NP) pseudostationary phase (PSP) for electrokinetic chromatography have been characterized. The anionic NPs have very low or no affinity for cationic solutes, but show significant interactions and retention based on hydrophobic interactions. Retention factors of alkyl-phenyl ketones increase linearly with the concentration of the NPs and have zero or near zero y-intercepts as expected for electrokinetic chromatography with non-micellar PSPs. The retention factors of these solutes and representative pharmaceuticals decrease logarithmically with increases in the concentration of ACN in the background electrolyte, as expected for reversed-phase retention. Linear solvation energy relationship analysis indicates that the NPs are less cohesive than would be expected for polymeric PSPs with similar structure but that the overall separation selectivity can be expected to be similar to polymer PSPs with similar backbone chemistry. The results indicate that the hydrophobic core of the NPs is non-cohesive and is highly accessible to solutes, whereas the ionic head groups are not as accessible and do not contribute substantially to retention or selectivity.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Palmer, CP (Dr Christopher Palmer)
UTAS Author:Hilder, EF (Professor Emily Hilder)
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:72210
Year Published:2011
Funding Support:Australian Research Council (FT0990521)
Web of Science® Times Cited:15
Deposited By:Chemistry
Deposited On:2011-08-24
Last Modified:2012-05-10

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