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Evidence of an antimicrobial-immunomodulatory role of Atlantic salmon cathelicidins during infection with Yersinia ruckeri
Citation
Bridle, A and Nosworthy, E and Polinski, M and Nowak, B, Evidence of an antimicrobial-immunomodulatory role of Atlantic salmon cathelicidins during infection with Yersinia ruckeri , Plos One , 6, (8) Article e23417. ISSN 1932-6203 (2011) [Refereed Article]
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Copyright Statement
Licenced under Creative Commons Attribution 2.5 Generic (CC BY 2.5) http://creativecommons.org/licenses/by/2.5/
DOI: doi:10.1371/journal.pone.0023417
Abstract
Cathelicidins are a family of antimicrobial peptides that act as effector molecules of the innate immune system with broadspectrum
antimicrobial properties. These evolutionary conserved cationic host-defence peptides are integral components
of the immune response of fish, which are generally believed to rely heavily on innate immune defences to invading
pathogens. In this study we showed that Atlantic salmon cathelicidin 1 and 2 (asCATH1 and asCATH2) stimulated peripheral
blood leukocytes increasing the transcription of the chemokine interleukin-8. Further, functional differences were identified
between the two cathelicidins. In the presence of serum, asCATH1 displayed greatly diminished host haemolytic activity,
while the constitutively expressed asCATH2 had no haemolytic activity with or without serum. These findings support our
hypothesis that fish cathelicidins exert their primary antimicrobial action at the site of pathogen invasion such as epithelial
surfaces. Further, we hypothesise that like their mammalian counterparts in the presence of serum they act as mediators of
the innate and adaptive immune response via the release of cytokines thus indirectly protecting against a variety of
pathogens. We highlight the importance of this immunomodulatory role from the involvement of asCATHs during an
infection with the fish pathogen Yersinia ruckeri. While we were able to demonstrate in vitro that asCATH1 and 2, possessed
direct microbicidal activity against the fish pathogen, Vibrio anguillarum, and a common gram negative bacterium,
Escherichia coli, little or no bactericidal activity was found against Y. ruckeri. The contribution of either asCATH in the
immune response or as a potential virulence factor during yersiniosis is highlighted from the increased expression of
asCATH1 and 2 mRNA during an in vivo challenge with Y. ruckeri. We propose that Atlantic salmon cathelicidins participate
in the interplay between the innate and adaptive immune systems via the release of cytokines enabling a more effective
response to invading pathogens.
Item Details
Item Type: | Refereed Article |
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Research Division: | Agricultural, Veterinary and Food Sciences |
Research Group: | Fisheries sciences |
Research Field: | Fish pests and diseases |
Objective Division: | Animal Production and Animal Primary Products |
Objective Group: | Fisheries - aquaculture |
Objective Field: | Aquaculture fin fish (excl. tuna) |
UTAS Author: | Bridle, A (Associate Professor Andrew Bridle) |
UTAS Author: | Nosworthy, E (Ms Elizabeth Nosworthy) |
UTAS Author: | Polinski, M (Mr Mark Polinski) |
UTAS Author: | Nowak, B (Professor Barbara Nowak) |
ID Code: | 72066 |
Year Published: | 2011 |
Web of Science® Times Cited: | 68 |
Deposited By: | NC Marine Conservation and Resource Sustainability |
Deposited On: | 2011-08-19 |
Last Modified: | 2017-07-26 |
Downloads: | 445 View Download Statistics |
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