Polymeric monoliths in capillary formats have been fabricated incorporating a gradient of charged functional groups along their length. Scanning capacitively coupled contactless conductivity detection (sC4D) was then used to measure the conductive response of the stationary phase and characterise the relative axial distribution of functional groups along the column length. Gradients of 2-acrylamido-2-methyl-1-propanesulphonic acid were prepared using either photografting methods or by filling a capillary column with segmented plugs of monomer mixtures each containing incrementally higher concentrations of the functional monomer. The utility of sC4D as a rapid and non-invasive tool for assessing the slope of a variety of gradient configurations is demonstrated. Repeatability of the sC4D measurements was <1.7% RSD. Columns with a gradient of covalently bonded iminodiacetic acid were also produced. Changes in the gradient slope were observed after chelation of copper on the stationary phase via a reduction of the conductive response. The effect upon the observed gradient profile of changing the comonomer composition during column fabrication was studied. © 2010 WILEY-VCH Verlag GmBH & Co. KGaA.

Item Type:	Refereed Article
Research Division:	Chemical Sciences
Research Group:	Analytical Chemistry
Research Field:	Separation Science
Objective Division:	Expanding Knowledge
Objective Group:	Expanding Knowledge
Objective Field:	Expanding Knowledge in the Chemical Sciences
Author:	Currivan, S (Dr Sinead Currivan)
Author:	Paull, B (Professor Brett Paull)
ID Code:	72032
Year Published:	2010
Web of Science® Times Cited:	12
Deposited By:	Research Division
Deposited On:	2011-08-18
Last Modified:	2015-11-17
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