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Evaluation of photografted charged sites within polymer monoliths in capillary columns using contactless conductivity detection

Citation

Connolly, D and O'Shea, V and Clark, P and O'Connor, B and Paull, B, Evaluation of photografted charged sites within polymer monoliths in capillary columns using contactless conductivity detection, Journal of Separation Science, 30, (17) pp. 3060-3068. ISSN 1615-9306 (2007) [Refereed Article]

DOI: doi:10.1002/jssc.200700365

Abstract

Capacitively coupled contactless conductivity detection (C 4D) is presented as a novel versatile means of visualising discrete zones of charged functional groups grafted onto polymer based monoliths. Monoliths were first formed within 100 μm UV-transparent fused silica capillaries. Photografting methods were subsequently used to graft a charged functional monomer, 2-acrylamido-2-methyl-1-propanesulfonic acid, onto discrete regions of the monolith using a photomask. Post-modification monolith evaluation involves scanning the C 4D detector along the length of the monolith to obtain a profile of the exact spatial location of grafted charged functionalities with millimetre accuracy. The methodology was extended to the visualisation of several zones of immobilised protein (bovine serum albumin) using photografted azlactone groups to enable covalent attachment of the protein to the monolith at precise locations along its length. In addition, the extent of non-specific binding of protein to the ungrafted regions of the monolith due to hydrophobic interactions could be monitored as an increase in background conductivity of the stationary phase. Finally, the technique was cross-validated using digital photography in combination with a UV light source by immobilising green fluorescent protein in discrete zones and comparing the results obtained using both complementary techniques. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Paull, B (Professor Brett Paull)
ID Code:72008
Year Published:2007
Web of Science® Times Cited:32
Deposited By:Research Division
Deposited On:2011-08-18
Last Modified:2011-08-18
Downloads:0

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