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Separation of nucleic acid precursors on an amphoteric surfactant modified monolith using combined eluent flow, pH and concentration gradient
Citation
Nesterenko, EP and Guivarc'h, C and Duffy, C and Paull, B, Separation of nucleic acid precursors on an amphoteric surfactant modified monolith using combined eluent flow, pH and concentration gradient, Journal of Separation Science, 30, (17) pp. 2910-2916. ISSN 1615-9306 (2007) [Refereed Article]
DOI: doi:10.1002/jssc.200700355
Abstract
An RP monolithic column coated with an amphoteric carboxybetaine type surfactant has been used with a combined triple eluent concentration, pH and flow gradient ion chromatography technique for the simultaneous separation of up to 18 nucleotides, nucleosides and nucleobases. The separation of up to eight precursors on a 1 cm long monolithic microcolumn using the combined gradient approach is also shown. The method was applied to the separation of the above nucleic acid precursors in perchloric acid extracts of yeastolates samples. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Chemical Sciences |
| Research Group: | Analytical Chemistry |
| Research Field: | Separation Science |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding Knowledge |
| Objective Field: | Expanding Knowledge in the Chemical Sciences |
| Author: | Paull, B (Professor Brett Paull) |
| ID Code: | 72003 |
| Year Published: | 2007 |
| Web of Science® Times Cited: | 5 |
| Deposited By: | Research Division |
| Deposited On: | 2011-08-18 |
| Last Modified: | 2011-08-18 |
| Downloads: | 0 |
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