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Effect of vaccination against yersiniosis on the relative percent survival, bactericidal and lysozyme response of Atlantic salmon, Salmo salar
Citation
Costa, AA and Leef, MJ and Bridle, AR and Carson, J and Nowak, BF, Effect of vaccination against yersiniosis on the relative percent survival, bactericidal and lysozyme response of Atlantic salmon, Salmo salar , Aquaculture: An International Journal Devoted to Fundamental Aquatic Food Resources, 315, (3-4) pp. 201-206. ISSN 0044-8486 (2011) [Refereed Article]
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DOI: doi:10.1016/j.aquaculture.2011.02.031
Abstract
The bacterium Yersinia ruckeri serovar O1b causes yersiniosis in Atlantic salmon, Salmo salar, in the southern
hemisphere. Despite vaccination this disease has resulted in significant hatchery losses in the Tasmanian
Atlantic salmon aquaculture industry. A poor response to vaccination in juveniles, 1–5 g, has lead to the
investigation of the suitability of the current formalin killed whole-cell vaccine Yersinivac-B. In this study
trypsin was added to the Yersinivac-B to expose the bacteria's protective O-antigen to make the vaccine more
immunogenic. At six weeks post vaccination, the effect of Yersinivac-B and the novel trypsinated Yersinivac-B
vaccine on body mucus lysozyme and mucus and serum bactericidal activity of fish was determined over a
48 h period following challenge with Y. ruckeri. Body and gill mucus lysozyme and mucus and serum
bactericidal activity was also determined in surviving fish at 10 weeks post Y. ruckeri challenge. Following the
challenge period of 14 days the trypsinated Yersinivac-B fish demonstrated a significantly higher percent
survival compared to the Yersinivac-B and control unvaccinated fish. Body mucus lysozyme concentration
was also significantly elevated at 8 h post challenge in the trypsinated Yersinivac-B fish compared to controls.
This variable however appears unlikely to play a significant role in protection as positive bactericidal activity
was not found in the mucus of any fish following challenge. Bactericidal activity was not observed in the
serum or mucus of any challenge survivors. At 8 h post challenge the trypsinated Yersinivac-B fish
demonstrated the highest serum bactericidal activity. However, the unvaccinated control fish also displayed
positive serum bactericidal activity despite being unlikely to have been previously exposed to Y. ruckeri. A
significantly higher gill mucus lysozyme concentration in control survivors compared to vaccinated fish
suggests that this response may be important in the protection of unvaccinated fish against yersiniosis. This
research has highlighted the potential use of trypsin to increase the efficacy of Yersinivac-B. It has also
contributed to better understanding of the role of humoral immune responses during a Y. ruckeri challenge.
Item Details
Item Type: | Refereed Article |
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Keywords: | yersiniosis, Yersinia ruckeri, Salmo salar, lysozyme, bacterial activity, vaccinations |
Research Division: | Agricultural and Veterinary Sciences |
Research Group: | Fisheries Sciences |
Research Field: | Fish Pests and Diseases |
Objective Division: | Animal Production and Animal Primary Products |
Objective Group: | Fisheries - Aquaculture |
Objective Field: | Aquaculture Fin Fish (excl. Tuna) |
UTAS Author: | Costa, AA (Ms Amanda Costa) |
UTAS Author: | Leef, MJ (Dr Melanie Leef) |
UTAS Author: | Bridle, AR (Dr Andrew Bridle) |
UTAS Author: | Nowak, BF (Professor Barbara Nowak) |
ID Code: | 71987 |
Year Published: | 2011 |
Web of Science® Times Cited: | 23 |
Deposited By: | NC Marine Conservation and Resource Sustainability |
Deposited On: | 2011-08-18 |
Last Modified: | 2017-11-03 |
Downloads: | 2 View Download Statistics |
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