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Strain characterization of potato virus S isolates from Tasmania, Australia
Citation
Lambert, SJ and Scott, JB and Pethybridge, SJ and Hay, FS, Strain characterization of potato virus S isolates from Tasmania, Australia, Plant Disease: An International Journal of Applied Plant Pathology, 96, (6) pp. 813-819. ISSN 0191-2917 (2012) [Refereed Article]
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Copyright Statement
Copyright 2012 The American Phytopathological Society
DOI: doi:10.1094/PDIS-07-11-0573
Abstract
Potato virus S (PVS) is prevalent within potato (Solanum tuberosum)
production worldwide. Traditionally, PVS has been split into two
strains, Ordinary (PVSO) and Andean (PVSA), based on reaction in
herbaceous indicator species such as Chenopodium quinoa. However,
recent research has identified further strain designations, such as PVSO-CS
(Ordinary and Chenopodium systemic). Forty-four isolates of PVS
were collected from potato seed lines in different geographical regions
within Tasmania, Australia. Isolates were initially characterized by
reactions in C. quinoa. Nineteen isolates were characterized as PVSO,
based on the development of local lesions and serological detection in
inoculated leaves only. Three isolates were identified as PVSA-like,
based on local lesion development in inoculated leaves, mild mottling
or chlorotic spots on noninoculated leaves, and serological detection in
both inoculated and noninoculated leaves. Thirteen isolates produced
no symptoms, and were detected serologically in inoculated leaves only (PVSO-like). Four isolates produced no symptoms but were detected
serologically in both inoculated and noninoculated leaves
(PVSA-like). Five isolates produced symptoms in inoculated leaves
only but were detected serologically in both inoculated and noninoculated
leaves (also PVSA-like). The ability of isolates to infect tomato
has also been used as a criterion to assist in PVS strain differentiation.
A subsample of isolates (n = 16) was unable to infect tomato 'Grosse
Lisse'. Seventeen isolates representative of these groupings based on
reactions in C. quinoa were also characterized by coat-protein
sequencing. Phylogenetic comparisons suggested that all isolates were
PVSO rather than PVSA. Therefore, whereas some of these PVS isolates
were systemic in C. quinoa, findings from this study suggest that
they were not PVSA, and that only PVSO and PVSO-CS isolates are present
in Tasmania. The implications of this finding for disease management
are discussed.
Item Details
Item Type: | Refereed Article |
---|---|
Keywords: | potato virus S, isolates, strain, Solanum tuberosum, Chenopodium quinoa, |
Research Division: | Agricultural, Veterinary and Food Sciences |
Research Group: | Horticultural production |
Research Field: | Horticultural crop protection (incl. pests, diseases and weeds) |
Objective Division: | Plant Production and Plant Primary Products |
Objective Group: | Horticultural crops |
Objective Field: | Field grown vegetable crops |
UTAS Author: | Lambert, SJ (Dr Susan Lambert) |
UTAS Author: | Scott, JB (Dr Jason Scott) |
UTAS Author: | Pethybridge, SJ (Dr Sarah Pethybridge) |
UTAS Author: | Hay, FS (Dr Frank Hay) |
ID Code: | 71806 |
Year Published: | 2012 |
Web of Science® Times Cited: | 9 |
Deposited By: | Agricultural Science |
Deposited On: | 2011-08-08 |
Last Modified: | 2013-05-13 |
Downloads: | 0 |
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