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Strain characterization of potato virus S isolates from Tasmania, Australia

Citation

Lambert, SJ and Scott, JB and Pethybridge, SJ and Hay, FS, Strain characterization of potato virus S isolates from Tasmania, Australia, Plant Disease: An International Journal of Applied Plant Pathology, 96, (6) pp. 813-819. ISSN 0191-2917 (2012) [Refereed Article]


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Copyright Statement

Copyright 2012 The American Phytopathological Society

DOI: doi:10.1094/PDIS-07-11-0573

Abstract

Potato virus S (PVS) is prevalent within potato (Solanum tuberosum) production worldwide. Traditionally, PVS has been split into two strains, Ordinary (PVSO) and Andean (PVSA), based on reaction in herbaceous indicator species such as Chenopodium quinoa. However, recent research has identified further strain designations, such as PVSO-CS (Ordinary and Chenopodium systemic). Forty-four isolates of PVS were collected from potato seed lines in different geographical regions within Tasmania, Australia. Isolates were initially characterized by reactions in C. quinoa. Nineteen isolates were characterized as PVSO, based on the development of local lesions and serological detection in inoculated leaves only. Three isolates were identified as PVSA-like, based on local lesion development in inoculated leaves, mild mottling or chlorotic spots on noninoculated leaves, and serological detection in both inoculated and noninoculated leaves. Thirteen isolates produced no symptoms, and were detected serologically in inoculated leaves only (PVSO-like). Four isolates produced no symptoms but were detected serologically in both inoculated and noninoculated leaves (PVSA-like). Five isolates produced symptoms in inoculated leaves only but were detected serologically in both inoculated and noninoculated leaves (also PVSA-like). The ability of isolates to infect tomato has also been used as a criterion to assist in PVS strain differentiation. A subsample of isolates (n = 16) was unable to infect tomato 'Grosse Lisse'. Seventeen isolates representative of these groupings based on reactions in C. quinoa were also characterized by coat-protein sequencing. Phylogenetic comparisons suggested that all isolates were PVSO rather than PVSA. Therefore, whereas some of these PVS isolates were systemic in C. quinoa, findings from this study suggest that they were not PVSA, and that only PVSO and PVSO-CS isolates are present in Tasmania. The implications of this finding for disease management are discussed.

Item Details

Item Type:Refereed Article
Keywords:potato virus S, isolates, strain, Solanum tuberosum, Chenopodium quinoa,
Research Division:Agricultural and Veterinary Sciences
Research Group:Horticultural Production
Research Field:Horticultural Crop Protection (Pests, Diseases and Weeds)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural Crops
Objective Field:Vegetables
Author:Lambert, SJ (Dr Susan Lambert)
Author:Scott, JB (Dr Jason Scott)
Author:Pethybridge, SJ (Dr Sarah Pethybridge)
Author:Hay, FS (Dr Frank Hay)
ID Code:71806
Year Published:2012
Web of Science® Times Cited:5
Deposited By:Agricultural Science
Deposited On:2011-08-08
Last Modified:2013-05-13
Downloads:0

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