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Visible light initiated polymerization of styrenic monolithic stationary phases using 470 nm light emitting diode arrays

Citation

Walsh, Z and Levkin, PA and Jain, V and Paull, B and Svec, F and Macka, M, Visible light initiated polymerization of styrenic monolithic stationary phases using 470 nm light emitting diode arrays, Journal of Separation Science, 33, (1) pp. 61-66. ISSN 1615-9306 (2010) [Refereed Article]

DOI: doi:10.1002/jssc.200900624

Abstract

Poly (styrene-co-divinylbenzene) monolithic stationary phases have been synthesized for the first time by photoinitated polymerization. An initiator composed of (+)-(S)camphorquinone/ethyl-4-dimethylaminobenzoate/N-methoxy-4-phenylpyridinium tetrafluoroborate was activated using a 470 nm light emitting diode array as the light source. Spatially controlled polymerization of styrenic monoliths has been achieved within specific sections of a 100 pm id polytetrafluoroethylene-coated fused-silica capillary using simple photo masking. The sharpness of the edges was confirmed by optical microscopy, while SEM was used to verify a typical porous, globular morphology. Flow resistance data were used to assess the permeability of the monoliths and they were found to have good flow through properties with a flow resistance of 0.725 MPa/cm at 1 mu L/min (water, 20 degrees C). Conductivity profiling along the length of the capillary was used to assess their lateral homogeneity. Monoliths which were axially rotated during polymerization were found to be homogeneous along the whole length of the capillary. The monolithic stationary phases were applied to the RP gradient separation of a mixture of proteins. Column fabrication showed excellent reproducibility with the retention factor (k) having a RSD value of 2.6% for the batch and less than 1.73% on individual columns.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Paull, B (Professor Brett Paull)
Author:Macka, M (Professor Mirek Macka)
ID Code:71634
Year Published:2010
Web of Science® Times Cited:27
Deposited By:Research Division
Deposited On:2011-07-26
Last Modified:2015-02-05
Downloads:0

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