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Development of microfluidic chips for heterogeneous receptor-ligand interaction studies


Goldberg, MD and Lo, RC and Abele, S and Macka, M and Gomez, FA, Development of microfluidic chips for heterogeneous receptor-ligand interaction studies, Analytical Chemistry, 81, (12) pp. 5095-5098. ISSN 0003-2700 (2009) [Refereed Article]

DOI: doi:10.1021/ac9006649


A simple microfluidic-based technique to quantitate the binding affinity between the glycopeptide antibiotics teicoplanin from Actinoplanes teicomyceticus and vancomycin from Streptomyces orientalis and 5-carboxyfluorescein-D-Ala-D-Ala-D-Ala (5-FAM-(DA)(3)) is described. In this work, (3-aminopropyl)triethoxysilane is used to modify the surfaces of a series of microchannels, and each channel is subsequently exposed to a solution of antibiotic for a few minutes. The antibiotic is retained after washing through electrostatic interactions, and die series of channels are subsequently exposed to an increasing concentration of 5TAM-(DA)(3) followed by washing to exclude any nonspecific binding. The extent of fluorescence is quantified using a microscope fitted with a CCD camera. The binding constants for the interaction of teicoplanin and vancomycin with the fluorescent peptide were determined to be 6.03 +/- 0.97 x 10(4) and 4.03 +/- 1.13 x 10(4) M(-1), respectively, in good agreement with previous data. The ease of quantifying the extent of interaction in this microchip technique may prove powerful for exploration of a myriad of receptor-ligand pairs.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Macka, M (Professor Mirek Macka)
ID Code:71631
Year Published:2009
Web of Science® Times Cited:12
Deposited By:Research Division
Deposited On:2011-07-25
Last Modified:2011-08-01

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