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UV-absorbance detector for HPLC based on a light-emitting diode


Schmid, S and Macka, M and Hauser, PC, UV-absorbance detector for HPLC based on a light-emitting diode, The Analyst, 133, (4) pp. 465-469. ISSN 0003-2654 (2008) [Refereed Article]

DOI: doi:10.1039/b715681b


A flow-through optical absorption detector for HPLC was constructed using a novel deep-UV light-emitting diode as radiation source with a peak emission wavelength of 255 nm. For measuring the transmitted intensity (a property correlated to Transmittance) a special UV-sensitive photodiode was employed. Besides the power source, no optical or electronic components other than an inexpensive operational amplifier and a few passive components were necessary. The performance of the detector was tested with three substances, namely nitrobenzene, benzoic acid and methyl benzoate, which were separated by gradient elution using an acetonitrile/water mixture and tetrabutylammonium hydrogensulfate as pH-buffer. Calibration curves for concentrations between 1.6 mu g(.)mL(-1) and 400 mu g(.)mL(-1) (nitrobenzene) and 8 mu g(.)mL(-1) and 2.5 mg(.)mL(-1) (benzoic acid and methyl benzoate) were determined and coefficients of determination, r(2), of 0.9945, 0.9972 and 0.9996 were obtained for quadratic curve fits for the 3 compounds respectively. Relative standard deviations (n = 7) for peak areas were determined as 0.35% (nitrobenzene, 80 mu g(.)mL(-1)), 0.27% (benzoic acid, 400 mu g(.)mL(-1)) and 0.83% (methyl benzoate, 200 mu g(.)mL(-1)). The lower limits of detection were found to be 750 ng(.)mL(-1), 5.8 mu g(.)mL(-1) and 12 mu g(.)mL(-1) for nitrobenzene, benzoic acid and methyl benzoate respectively.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Macka, M (Professor Mirek Macka)
ID Code:71625
Year Published:2008
Web of Science® Times Cited:30
Deposited By:Research Division
Deposited On:2011-07-25
Last Modified:2011-07-25

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