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Spine-Scale Reorientation in Apedinella radians (Pedinellales, Chrysophyceae): the Microarchitecture and Immunocytochemistry of the Associated Cytoskeleton

journal contribution
posted on 2023-05-17, 06:41 authored by Anthony KoutoulisAnthony Koutoulis, McFadden, GI, Wetherbee, R
Motile unicells of Apedinella radians have the extraordinary ability to instantaneously reorient six elongate spine-scales located on the cell surface. Extracellular striated fibrous connectors (termed microligaments) attach spine-scales to discrete regions of the plasma membrane underlain by intricate cytoplasmic plaques. A complex cytoskeleton is associated with the plaques and appears responsible for spine-scale movement. Three cytoskeletal proteins have thus far been identified by immunofluorescence using anti-tubulin, anti-actin, and anti-centrin. The three-dimensional configuration of the cytoskeleton has been established and consists of filamentous bundles of actin and centrin which form stellate systems interconnecting the plaques. Additionally, there is a network of microtubular triads which originate on the surface of the nuclear envelope and subtend the plasma membrane and also support several tentacular protrusions. It is proposed that contraction of the actin and/or centrin filamentous bundles is responsible for the reorientation of the spinescales.

History

Publication title

Protoplasma: An International Journal of Cell Biology

Volume

147

Pagination

25-41

ISSN

0033-183X

Department/School

School of Natural Sciences

Publisher

Springer-Verlag Wien

Place of publication

Sachsenplatz 4-6, Po Box 89, Vienna, Austria, A-1201

Rights statement

The final publication is available at http://www.springerlink.com

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the biological sciences

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