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A scale associated protein of Apedinella radians (Pedinellophyceae) and its possible role in the adhesion of surface components
journal contribution
posted on 2023-05-17, 06:41 authored by Anthony KoutoulisAnthony Koutoulis, Ludwig, M, Wetherbee, RMonoclonal antibodies have been generated against cell surface components of the unicellular phytoflagellate Apedinella radians (Pedinellophyceae). One monoclonal antibody, designated Arg 1E5/1B1, labels a scale associated protein (SAP) of 145 kDa. Immunofluorescence microscopy of whole cells as well as immunoelectron microscopy of whole cell mounts and thin sections using Arg 1E5/1B1 have shown that the SAP is located on the proximal surface of body scales and spine-scales. Its specific location suggests that the SAP may play a role in the adhesion of these surface components to the cell membrane and/or to one another. The potential of monoclonal antibody Arg 1E5/1B1 as a tool to study cell surface morphogenesis and the role of the endomembrane system in A. radians is discussed.
History
Publication title
Journal of Cell ScienceVolume
104Pagination
391-398ISSN
0021-9533Department/School
School of Natural SciencesPublisher
Company Of Biologists LtdPlace of publication
Bidder Building Cambridge Commercial Park Cowley Rd, Cambridge, England, Cambs, Cb4 4DlRights statement
© The Company of Biologists Ltd 2011Repository Status
- Restricted