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Monoclonal antibodies have been generated against cell surface components of the unicellular phytoflagellate Apedinella radians (Pedinellophyceae). One monoclonal antibody, designated Arg 1E5/1B1, labels a scale associated protein (SAP) of 145 kDa. Immunofluorescence microscopy of whole cells as well as immunoelectron microscopy of whole cell mounts and thin sections using Arg 1E5/1B1 have shown that the SAP is located on the proximal surface of body scales and spine-scales. Its specific location suggests that the SAP may play a role in the adhesion of these surface components to the cell membrane and/or to one another. The potential of monoclonal antibody Arg 1E5/1B1 as a tool to study cell surface morphogenesis and the role of the endomembrane system in A. radians is discussed.

Item Type:	Refereed Article
Research Division:	Biological Sciences
Research Group:	Plant biology
Research Field:	Phycology (incl. marine grasses)
Objective Division:	Expanding Knowledge
Objective Group:	Expanding knowledge
Objective Field:	Expanding knowledge in the biological sciences
UTAS Author:	Koutoulis, A (Professor Anthony Koutoulis)
ID Code:	70913
Year Published:	1993
Web of Science® Times Cited:	2
Deposited By:	Research Division
Deposited On:	2011-07-05
Last Modified:	2011-10-12
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