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Odd-chain polyunsaturated fatty acids in thraustochytrids


Lee Chang, KJ and Mansour, MP and Dunstan, GA and Blackburn, SI and Koutoulis, A and Nichols, PD, Odd-chain polyunsaturated fatty acids in thraustochytrids , Phytochemistry: International Journal of Plant Biochemistry, 72, (11-12) pp. 1460-1465. ISSN 0031-9422 (2011) [Refereed Article]

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DOI: doi:10.1016/j.phytochem.2011.04.001


A series of unusual odd-chain fatty acids (OC-FA) were identified in two thraustochytrid strains, TC 01 and TC 04, isolated from waters off the south east coast of Tasmania, Australia. FA compositions were determined by capillary GC and GC–MS, with confirmation of polyunsaturated fatty acids (PUFA) structure performed by analysis of 4,4-dimethyloxazoline derivatives. PUFA constituted 68–74% of the total FA, with the essential PUFA; eicosapentaenoic acid (20:5ω3, EPA), arachidonic acid (20:4ω6, AA) and docosahexaenoic acid (22:6ω3, DHA), accounting for 42–44% of the total FA. High proportions of the saturated OC-FA 15:0 (7.1% in TC 01) and 17:0 (6.2% in TC 04) were detected. The OC-FA 17:1ω8 was present at 2.8% in TC 01. Of particular interest, the C21 PUFA 21:5ω5 and 21:4ω7 were detected at 3.5% and 4.1%, respectively, in TC 04. A proposed biosynthesis pathway for these OC-PUFA is presented. It is possible that the unsaturated OC-PUFA found previously in a number of marine animals were derived from dietary thraustochytrids and they could be useful biomarkers in environmental and food web studies.

Item Details

Item Type:Refereed Article
Keywords:thraustochytrids, odd-carbon fatty acids, polyenoic fatty acids, food web studies
Research Division:Biological Sciences
Research Group:Biochemistry and cell biology
Research Field:Biochemistry and cell biology not elsewhere classified
Objective Division:Environmental Management
Objective Group:Terrestrial systems and management
Objective Field:Assessment and management of terrestrial ecosystems
UTAS Author:Lee Chang, KJ (Mr Kim Lee Chang)
UTAS Author:Koutoulis, A (Professor Anthony Koutoulis)
ID Code:70867
Year Published:2011
Web of Science® Times Cited:33
Deposited By:Plant Science
Deposited On:2011-07-05
Last Modified:2017-11-03

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