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Temporal transcriptomic profiling reveals cellular targets that govern survival in HOCl-mediated neuronal apoptosis


Yap, YW and Chen, Minghui Jessica and Choy, MS and Peng, ZF and Whiteman, M and Manikandan, J and Melendez, AJ and Cheung, NS, Temporal transcriptomic profiling reveals cellular targets that govern survival in HOCl-mediated neuronal apoptosis, Life Sciences, 87, (15-16) pp. 457-467. ISSN 0024-3205 (2010) [Refereed Article]

DOI: doi:10.1016/j.lfs.2010.08.011


Aims: With the identification of hypochlorous acid (HOCl) as a biomarker in diseased brains and endogenous detection of its modified proteins, HOCl might be implicated in the development of neurodegenerative disorders. However, its effect on neuronal cell death has not yet been investigated at gene expression level. Main methods: Therefore, DNA microarray was performed for screening of HOCl-responsive genes in primary mouse cortical neurons. Neurotoxicity caused by physiological relevant HOCl (250 μM) exhibited several biochemical markers of apoptosis. Key findings: The biological processes affected during HOCl-mediated apoptosis included cell death, response to stress, cellular metabolism, and cell cycle. Among them, mRNAs level of cell death and stress response genes were up-regulated while expression of metabolism and cell cycle genes were down-regulated. Significance: Our results showed, for the first time, that HOCl induces apoptosis in cortical neurons by upregulating apoptotic genes and gene expression of stress response such as heat shock proteins and antioxidant proteins were enhanced to provide protection. These data form a foundation for the development of screening platforms and define targets for intervention in HOCl neuropathologies where HOCl-mediated injury is causative.

Item Details

Item Type:Refereed Article
Keywords:Hypochlorous acid; Oxidative stress; Apoptosis; Neurodegenerative disorders
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Neurology and neuromuscular diseases
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Chen, Minghui Jessica (Ms Minghui Chen)
UTAS Author:Cheung, NS (Dr Nam Cheung)
ID Code:70497
Year Published:2010
Web of Science® Times Cited:8
Deposited By:Research Division
Deposited On:2011-06-23
Last Modified:2011-06-24

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