eCite Digital Repository

Identification of a cell-surface located protein with growth at low pH in Bifidobacterium animalis (syn. lactis) Strain Bb12

Citation

Pepper, S and Britz, ML, Identification of a cell-surface located protein with growth at low pH in Bifidobacterium animalis (syn. lactis) Strain Bb12, 7th International Symposium on Industrial Microbiology & Technology, 1-3 July 2010, Melbourne, Australia (2010) [Conference Extract]


Preview
PDF
Pending copyright assessment - Request a copy
92Kb
  

Abstract

Bifidobacterium animalis (syn. lactis) has been used as a starter culture in several manufactured product lines including yoghurts and anti-diarrhea tablets. As a starter culture it has been selected through a series of mutations and propogations to make it suitable for use in dairy products. This strain was studied to determine the affect of a sudden decrease in pH or continuous exposure to low pH.

Protein profiles of membrane and cell-surface associated fractions, for growth at both neutral pH (7.0) and mildly acidic conditions were essentially identical. At pH 5.0 an increase was noted in the density of a band around 33kOa. The same band was markedly increased during growth at pH 4.0. A 14 fold increase was observed in the relative amount of the protein present in cultures grown at pH 4.0.

Marked up-regulation of a protein of a protein of approximately 33kOa was a feature of 50S-PAGE gels of cells of Bifidobacterium animalis (syn. lactis) strain VUP Bb 12 grown under strongly acidic conditions.

Item Details

Item Type:Conference Extract
Keywords:Bifidobacterium animalis, Bifidobacterium lactis, cell curface protein, starter culture, yoghurt
Research Division:Engineering
Research Group:Food Sciences
Research Field:Food Sciences not elsewhere classified
Objective Division:Manufacturing
Objective Group:Dairy Products
Objective Field:Dairy Products not elsewhere classified
Author:Britz, ML (Professor Margaret Britz)
ID Code:70211
Year Published:2010
Deposited By:Agricultural Science
Deposited On:2011-06-08
Last Modified:2012-11-13
Downloads:1 View Download Statistics

Repository Staff Only: item control page