Pepper, S and Britz, ML, Identification of a cell-surface located protein with growth at low pH in Bifidobacterium animalis (syn. lactis) Strain Bb12, 7th International Symposium on Industrial Microbiology & Technology, 1-3 July 2010, Melbourne, Australia (2010) [Conference Extract]
Bifidobacterium animalis (syn. lactis) has been used as a starter culture in several manufactured product lines including yoghurts and anti-diarrhea tablets. As a starter culture it has been selected through a series of mutations and propogations to make it suitable for use in dairy products. This strain was studied to determine the affect of a sudden decrease in pH or continuous exposure to low pH.
Protein profiles of membrane and cell-surface associated fractions, for growth at both neutral pH (7.0) and mildly acidic conditions were essentially identical. At pH 5.0 an increase was noted in the density of a band around 33kOa. The same band was markedly increased during growth at pH 4.0. A 14 fold increase was observed in the relative amount of the protein present in cultures grown at pH 4.0.Marked up-regulation of a protein of a protein of approximately 33kOa was a feature of 50S-PAGE gels of cells of Bifidobacterium animalis (syn. lactis) strain VUP Bb 12 grown under strongly acidic conditions.
|Item Type:||Conference Extract|
|Keywords:||Bifidobacterium animalis, Bifidobacterium lactis, cell curface protein, starter culture, yoghurt|
|Research Group:||Food Sciences|
|Research Field:||Food Sciences not elsewhere classified|
|Objective Group:||Dairy Products|
|Objective Field:||Dairy Products not elsewhere classified|
|Author:||Britz, ML (Professor Margaret Britz)|
|Deposited By:||Agricultural Science|
|Downloads:||1 View Download Statistics|
Repository Staff Only: item control page