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Determination of Dietary Fiber and Carbohydrate Composition of Foods by a Modified Version of the Englyst Method

Citation

Lichon, MJ and James, KW, Determination of Dietary Fiber and Carbohydrate Composition of Foods by a Modified Version of the Englyst Method, Journal of AOAC International, 79, (1) pp. 54-61. ISSN 1060-3271 (1996) [Refereed Article]

Abstract

A modified version of the Englyst procedure for determination of dietary fiber and carbohydrate fractions of foods is described. The method is based on hydrolysis of separated fractions and capillary gas chromatographic (GC) analysis of derivatized monomer sugars, UV photometry of uronic acids, gravimetric determination of residue, and analysis of nitrogen content of the residue by using a C, H, N elemental analyzer. Modifications of fractionation, derivatization, and GC procedures are discussed. Rigorous sample preparation is required for reproducibility of 200 mg test portions. Contami- nants interfering with chromatography must be minimized. Results for reference samples (wheat bran and Buerre Bosc pears) and white and wholemeal bread agree with literature values.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Analytical Chemistry not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Lichon, MJ (Mr Michael John Lichon)
ID Code:7020
Year Published:1996
Web of Science® Times Cited:4
Deposited By:Plant Science
Deposited On:1996-08-01
Last Modified:2011-08-16
Downloads:0

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